OlsÉn KH, Grahn M, Lohm J, Langefors Å
Department of Environmental Toxicology, Uppsala University
Anim Behav. 1998 Aug;56(2):319-327. doi: 10.1006/anbe.1998.0837.
Kin recognition and discrimination are thought to occur in several species of various taxonomic groups. In salmonid fish, juveniles can discriminate between odours of siblings and nonsiblings from the same population even if the odour donors and the test fish have been reared separately since fertilization. This indicates that some genetic factor is important in the recognition process. The mechanisms behind kin recognition and discrimination have not yet been described. In the present study, we performed fluviarium tests to examine whether kin recognition and discrimination in juvenile Arctic charr are influenced by the major histocompatibility complex (MHC). Prior to the fluviarium tests, exon 2 of an MHC class II B gene in charr was analysed with denaturing gradient gel electrophoresis (DGGE) and individual genotypes were determined. In the fluviarium, when fish had the choice between water scented by an MHC identical sibling and a sibling with a different MHC genotype they preferred water from identical siblings. Moreover, water scented by an MHC different sibling was preferred to water from an MHC different nonsibling. However, we observed no discrimination when the test fish shared one allele with the nonsibling donor but no alleles with the sibling donor. Our results indicate that the MHC has a significant influence on the odours used for kin recognition and discrimination in juvenile Arctic charr.Copyright 1998 The Association for the Study of Animal Behaviour
亲缘识别和区分被认为存在于多个分类群的几种物种中。在鲑科鱼类中，即使气味供体和受试鱼自受精后就分开饲养，幼鱼也能区分来自同一群体的兄弟姐妹和非兄弟姐妹的气味。这表明某些遗传因素在识别过程中很重要。亲缘识别和区分背后的机制尚未得到描述。在本研究中，我们进行了水槽试验，以检验幼年北极红点鲑的亲缘识别和区分是否受主要组织相容性复合体（MHC）的影响。在水槽试验之前，用变性梯度凝胶电泳（DGGE）分析了红点鲑MHC II类B基因的外显子2，并确定了个体基因型。在水槽中，当鱼在由MHC相同的兄弟姐妹和具有不同MHC基因型的兄弟姐妹散发气味的水之间进行选择时，它们更喜欢来自相同兄弟姐妹的水。此外，由MHC不同的兄弟姐妹散发气味的水比由MHC不同的非兄弟姐妹散发气味的水更受青睐。然而，当受试鱼与非兄弟姐妹供体共享一个等位基因但与兄弟姐妹供体没有等位基因时，我们没有观察到区分现象。我们的结果表明，MHC对幼年北极红点鲑用于亲缘识别和区分的气味有显著影响。版权所有1998年动物行为研究协会
