Frantzen M A, Silk J B, Ferguson J W, Wayne R K, Kohn M H
Department of Zoology and Entomology, University of Pretoria, South Africa.
Mol Ecol. 1998 Oct;7(10):1423-8. doi: 10.1046/j.1365-294x.1998.00449.x.
We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 degrees C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
我们评估了四种用于保存粪便样本以进行DNA分析的方法的相对有效性。对从自由放养的狒狒身上采集的新鲜粪便样本进行的PCR检测表明，扩增成功率取决于保存方法、PCR产物大小以及检测的是核DNA还是线粒体DNA。保存在二甲基亚砜/乙二胺四乙酸/三羟甲基氨基甲烷/盐溶液（DETs）中对保存核DNA最为有效，但保存在70%乙醇中、在-20摄氏度下冷冻以及干燥对线粒体DNA和短（<200 bp）核DNA片段的效果大致相同。由于粪便DNA会被稀释和降解，可能需要从粪便中反复提取，并且应使用短核标记进行基因分型。对分子粪便学研究的综述进一步表明，每个个体应收集三到六份粪便。
