Sternbauer K, Luthman J, Hänni A, Jacobsson S O
Department of Ruminant Medicine and Veterinary Epidemiology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala University, Sweden.
Acta Vet Scand. 1998;39(2):281-9. doi: 10.1186/BF03547800.
Hyperinsulinemic, euglycemic clamp tests were performed on calves before and after clenbuterol treatment. Clenbuterol was given as 2 intramuscular injections with an interval of about 12 h. The dose used was 1 microgram/kg b.w. The treatment resulted in increased plasma levels of insulin and glucose. The results of the clamp tests showed that clenbuterol induced a transient decrease in insulin sensitivity. Both insulin mediated glucose disposal (M), expressed as mumol/kg live b.w./min. and the M/I-index (M divided by the average insulin concentration at steady state) were significantly reduced after treatment. The effect of clenbuterol on carbohydrate metabolism seemed to be rather short-lived, since significant changes occurred only in animals treated 5-6 h prior to the test. According to the literature, the metabolic effects of clenbuterol have been studied only after the high doses used for growth promoting purposes. The results from the present study showed that similar changes occur also after doses at the therapeutic level. The hyperinsulinemic, euglycemic clamp test was considered to be a valuable tool for the study of insulin sensitivity in cattle.
在小牛进行克伦特罗治疗前后，进行了高胰岛素-正血糖钳夹试验。克伦特罗通过两次肌肉注射给药，间隔约12小时。使用的剂量为1微克/千克体重。治疗导致血浆胰岛素和葡萄糖水平升高。钳夹试验结果表明，克伦特罗导致胰岛素敏感性短暂下降。治疗后，以微摩尔/千克活体重/分钟表示的胰岛素介导的葡萄糖处置（M）以及M/I指数（M除以稳态时的平均胰岛素浓度）均显著降低。克伦特罗对碳水化合物代谢的影响似乎相当短暂，因为仅在试验前5-6小时接受治疗的动物中出现了显著变化。根据文献，仅在用于促进生长目的的高剂量之后研究了克伦特罗的代谢作用。本研究结果表明，在治疗水平的剂量之后也会出现类似变化。高胰岛素-正血糖钳夹试验被认为是研究牛胰岛素敏感性的有价值工具。
