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基于酶联免疫吸附测定法(ELISA)检测初乳中抗体,对芬兰西部猪群中猪肺炎支原体的流行情况进行研究。

The prevalence of Mycoplasma hyopneumoniae in pig herds in western Finland based on the demonstration of antibodies in colostrum by ELISA.

作者信息

Rautiainen E

机构信息

National Veterinary and Food Research Institute, Regional Laboratory, Seinäjoki, Finland.

出版信息

Acta Vet Scand. 1998;39(3):325-30. doi: 10.1186/BF03547780.

Abstract

Swine enzootic pneumonia causes heavy economical losses in fattening herds. The aim of this study was to get an estimate of the prevalence of Mycoplasma hyopneumoniae in the province of Vaasa in Western Finland. There were 112 farrowing herds randomly chosen for a survey. Farmers were asked to milk colostrum samples from every sow. Owners of 22 herds did not send enough samples for a herd diagnosis. Altogether 1773 samples (mean 18.1 samples/herd) were analysed in an indirect ELISA for antibodies to Mycoplasma hyopneumoniae. Thirty-five herds were concluded to be infected with Mycoplasma hyopneumoniae. In these herds the mean prevalence of samples with antibodies was 16.3% (range 2.3%-70.0%, median 8.6%). The infected herds were significantly larger than the rest of the herds (no. of sows 27.0 vs. 18.4 respectively). The true prevalence of Mycoplasma hyopneumoniae infection in the province of Vaasa was estimated to be somewhat lower than the apparent prevalence of 39%. The application of colostrum serology is discussed.

摘要

猪地方性肺炎给育肥猪群造成了巨大的经济损失。本研究的目的是估算芬兰西部瓦萨省猪肺炎支原体的流行率。随机选择了112个产仔猪群进行调查。要求养殖户采集每头母猪的初乳样本。22个猪群的养殖户未提供足够的样本用于猪群诊断。总共1773份样本(平均每个猪群18.1份样本)通过间接ELISA法分析了猪肺炎支原体抗体。得出结论,有35个猪群感染了猪肺炎支原体。在这些猪群中,有抗体样本的平均流行率为16.3%(范围2.3%-70.0%,中位数8.6%)。感染猪群明显比其他猪群规模大(母猪数量分别为27.0头和18.4头)。据估计,瓦萨省猪肺炎支原体感染的实际流行率略低于39%的表观流行率。文中还讨论了初乳血清学检测的应用。

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本文引用的文献

1
Humoral immune responses to Mycoplasma hyopneumoniae in sows and offspring following an outbreak of mycoplasmosis.
Vet Microbiol. 1998 Feb 28;60(2-4):193-205. doi: 10.1016/s0378-1135(98)00155-2.
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A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae.
Res Vet Sci. 1994 May;56(3):338-45. doi: 10.1016/0034-5288(94)90151-1.
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Actinobacillus pleuropneumoniae serotype-2 antibodies in sow colostrum in Finnish pig-health-scheme herds.
Zentralbl Veterinarmed B. 1994 Nov;41(9):567-73. doi: 10.1111/j.1439-0450.1994.tb00265.x.

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