Levonen K, Veijalainen P, Seppänen J
National Veterinary and Food Research Institute, Helsinki, Finland.
Zentralbl Veterinarmed B. 1994 Nov;41(9):567-73. doi: 10.1111/j.1439-0450.1994.tb00265.x.
A direct ELISA with phenol-extracted antigen for Actinobacillus pleuropneumoniae serotype 2 was developed. The test was specific when tested with rabbit antisera prepared against different A. pleuropneumoniae serotypes. It had better-than-moderate repeatability and it made a clear distinction between positive and negative samples. A total of 5477 colostrum samples from breeding sows from herds participating in the Finnish Pig-health Scheme were tested using the ELISA test. A total of 1307 positive samples were found in 129 out of 154 herds, thus indicating that most of the disease-control herds in Finland are infected with A. pleuropneumoniae serotype 2. These infections were almost entirely subclinical.