Ueyama T
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Japan.
Kurume Med J. 1998;45(3):271-8. doi: 10.2739/kurumemedj.45.271.
Intrasplenic heterotransplantation of infantile testis is a conventional method to produce testicular tumors in adult castrated rodents. To avoid possible immunological interferences in the tumorigenic process in the allografted testis, the testicular tissues were autografted into the spleen of 88 castrated Wistar strain rats. Intrasplenic tumors developed in 64 animals (73% incidence) during an experimental period of 24 months, and 91% of these were histologically categorized as gonadal stromal tumors including Sertoli cell tumors, Leydig cell tumors, mixed forms of these and granulosa cell tumors. Although radioimmunoassay revealed elevated levels of serum sex-steroid hormones in 19 tumor-bearing rats, the levels of the serum gonadotropins were not increased. The results indicate that the intrasplenic testicular cells can develop into both Sertoli and granulosa type tumors. Intrasplenic autografting is a feasible and reproducible method to induce testicular gonadal stromal tumors with a high incidence, and thus deserves further attention.
婴儿睾丸脾内异体移植是在成年去势啮齿动物中产生睾丸肿瘤的传统方法。为避免同种异体移植睾丸在致瘤过程中可能出现的免疫干扰，将睾丸组织自体移植到88只去势的Wistar大鼠的脾脏中。在24个月的实验期内，64只动物（发生率73%）出现了脾内肿瘤，其中91%在组织学上被归类为性腺间质肿瘤，包括支持细胞瘤、间质细胞瘤、它们的混合形式以及颗粒细胞瘤。尽管放射免疫分析显示19只荷瘤大鼠血清性类固醇激素水平升高，但血清促性腺激素水平并未升高。结果表明，脾内睾丸细胞可发展为支持细胞型和颗粒细胞型肿瘤。脾内自体移植是一种可行且可重复的方法，可诱导高发生率的睾丸性腺间质肿瘤，因此值得进一步关注。
