Nishida T, Sugiyama T, Kataoka A, Ueyama T, Yakushiji M
Department of Obstetrics and Gynecology, Kurume University, School of Medicine, 67 Asahi-machi, Kurume, 830, Japan.
Oncol Rep. 1998 Jan-Feb;5(1):161-3.
To produce malignant granulosa cell tumors, ovarian tissue containing 7,12-dimethylbenz[a]anthracene (DMBA) was autografted in the spleen of 20 castrated rats. Adenocarcinoma developed in 8 rats within 6 months from the transplantation. Granulosa cell tumor arose from the intrasplenic graft, in 6 rats at the 14th month. The neoplastic cells were diffusely arranged in sheet showing focal luteinization. In 3 cases extrasplenic spreads were found on the peritoneal surface. Since malignant ovarian tumors seldom develop by intrasplenic grafting alone, the DMBA treatment may contribute to the occurrence of malignant granulosa cell tumor.
为了产生恶性颗粒细胞瘤，将含有7,12-二甲基苯并[a]蒽（DMBA）的卵巢组织自体移植到20只去势大鼠的脾脏中。移植后6个月内，8只大鼠发生腺癌。14个月时，6只大鼠的脾内移植物出现颗粒细胞瘤。肿瘤细胞呈弥漫性片状排列，可见局灶性黄体化。3例在腹膜表面发现脾外转移。由于单纯脾内移植很少发生恶性卵巢肿瘤，DMBA处理可能有助于恶性颗粒细胞瘤的发生。
