Nishida T, Ueyama T, Sugiyama T, Kataoka A, Yakushiji M
Department of Obstetrics and Gynecology, Kurume University, School of Medicine, 67 Asahi-machi, Kurume, 830, Japan.
Oncol Rep. 1998 Jan-Feb;5(1):157-9.
Intrasplenic heterotransplantation of infantile testis is a conventional method to produce testicular tumors in adult castrated rodents. To avoid possible immunological interference in the tumorigenic process in allografted testis, the parenchyma of testis of Wistar strain rats aged 7 weeks was injected into the spleen of the same rat after bilateral gonadectomy. In 17 of 20 animals testicular neoplasms, resembling gonadal stromal cell tumors in human, developed from the intrasplenic gonadal tissue in the 14th month following transplantation. This autografting technique is feasible to induce testicular neoplasms in granulosa cell tumor category in high incidence, and thus deserves further attention.
婴儿睾丸脾内异位移植是在成年去势啮齿动物中诱发睾丸肿瘤的传统方法。为避免同种异体移植睾丸在致瘤过程中可能出现的免疫干扰，对7周龄的Wistar品系大鼠进行双侧性腺切除后，将其睾丸实质注射到同一只大鼠的脾脏中。在20只动物中，有17只在移植后第14个月时，脾内性腺组织发生了类似于人类性腺间质细胞瘤的睾丸肿瘤。这种自体移植技术可高发病率地诱发颗粒细胞瘤类别的睾丸肿瘤，因此值得进一步关注。
