粗糙脉孢菌NAD糖水解酶的自动ADP核糖基化作用。
Auto-ADP-ribosylation of NAD glycohydrolase from Neurospora crassa.
作者信息
Cho Y S, Han M K, Kwark O S, Phoe M S, Cha Y S, An N H, Kim U H
机构信息
Department of Biochemistry, Chonbuk National University Medical School, Chonju, South Korea.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 1998 May;120(1):175-81. doi: 10.1016/s0305-0491(98)10006-8.
NAD glycohydrolase (NADase; EC 3.2.2.5) is an enzyme that catalyzes hydrolysis of NAD to produce ADP-ribose and nicotinamide. We recently demonstrated that self-inactivation of NADase from rabbit erythrocytes was due to an auto-ADP-ribosylation. In the present study, a mechanism of self-inactivation of NADase from Neurospora crassa by its substrate was investigated by using intact mycelia of N. crassa and purified NADase, which had molecular characteristics different from mammalian NADases. The results suggested that inactivation of NADase from N. crassa was also due to an auto-ADP-ribosylation. These findings indicate that the auto-modification of NADase is one of the universal phenomena to regulate enzyme functions.
NAD 糖水解酶（NADase；EC 3.2.2.5）是一种催化 NAD 水解生成 ADP - 核糖和烟酰胺的酶。我们最近证明，兔红细胞 NADase 的自失活是由于自动 ADP - 核糖基化。在本研究中，通过使用与哺乳动物 NADase 具有不同分子特征的粗糙脉孢菌完整菌丝体和纯化的 NADase，研究了粗糙脉孢菌 NADase 被其底物自失活的机制。结果表明，粗糙脉孢菌 NADase 的失活也是由于自动 ADP - 核糖基化。这些发现表明，NADase 的自动修饰是调节酶功能的普遍现象之一。
Zotero 插件