Poklis A, Poklis J L, Trautman D, Treece C, Backer R, Harvey C M
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0165, USA.
J Anal Toxicol. 1998 Oct;22(6):537-40. doi: 10.1093/jat/22.6.537.
The case history and toxicological findings of a fatal suicidal valproic acid overdose are presented. Valproic acid concentrations were determined in body tissues and fluids by gas-liquid chromatography (GLC) following both direct extraction and the method of standards addition and quantitative fluorescence polarization immunoassay. The quantitative results obtained by the three procedures were in good agreement. Qualitative identification of valproic acid as its methylated derivative was by ion-trap gas chromatography-mass spectrometry. Toxicological analysis by direct extraction GLC yielded the following valproate concentrations (mg/mL or mg/kg): blood, 1050; bile, 713; brain, 510; heart, 670; kidney, 1580; liver, 985; and vitreous, 516. A total of 15.1 g of valproate was recovered in the stomach contents. These findings far exceed those associated with valproate therapy and are similar to the limited valproate disposition data reported in prior fatal overdoses.
本文报告了一例因自杀过量服用丙戊酸致死的病例病史及毒理学发现。通过直接萃取、标准加入法以及定量荧光偏振免疫分析法，利用气液色谱法（GLC）测定了人体组织和体液中的丙戊酸浓度。三种方法得到的定量结果吻合良好。通过离子阱气相色谱 - 质谱法对丙戊酸的甲基化衍生物进行了定性鉴定。直接萃取GLC法进行的毒理学分析得出以下丙戊酸盐浓度（mg/mL或mg/kg）：血液，1050；胆汁，713；脑，510；心脏，670；肾脏，1580；肝脏，985；玻璃体液，516。胃内容物中总共回收了15.1 g丙戊酸盐。这些发现远远超过了丙戊酸盐治疗相关的浓度，且与先前致命过量服用病例中报告的有限的丙戊酸盐处置数据相似。
