Vila J, Gene A, Vargas M, Gascon J, Latorre C, Jimenez de Anta M T
Department of Microbiology, Hospital Clinic, University of Barcelona, School of Medicine, Spain.
J Med Microbiol. 1998 Oct;47(10):889-91. doi: 10.1099/00222615-47-10-889.
Escherichia coli strains associated with diarrhoeal disease have been classified into several types according to the pathogenic mechanism. Among these, enteroaggregative E. coli strains (EAggEC) have been associated with persistent childhood diarrhoea. Some strains of EAggEC produce a heat-stable toxin (EAST-1) that differs from others described previously. The main goal of this case-control study was to determine the prevalence of EAggEC and EAST-1-producing E. coli strains as a cause of diarrhoea in children in Spain and to study their in-vitro susceptibility to 21 antimicrobial agents. In the case group (115 children) 22 (19%) isolates and four (3.5%) isolates were EAST-1-producing E. coli and EAggEC, respectively, whereas in the control group (79 children) four (5%) isolates produced EAST-1 (p = 0.005) and three (3.8%) isolates were EAggEC. The present study suggests that EAST-1-producing E. coli strains are associated with diarrhoeal diseases in Spanish children, whereas EAggEC strains are not. Moreover, EAST-1-producing E. coli strains showed a high susceptibility to all the antimicrobial agents tested except for ampicillin.
根据致病机制，与腹泻病相关的大肠杆菌菌株已被分为几种类型。其中，肠集聚性大肠杆菌菌株（EAggEC）与儿童持续性腹泻有关。一些EAggEC菌株产生一种与先前描述的其他毒素不同的热稳定毒素（EAST-1）。本病例对照研究的主要目的是确定EAggEC和产EAST-1大肠杆菌菌株作为西班牙儿童腹泻病因的患病率，并研究它们对21种抗菌药物的体外敏感性。在病例组（115名儿童）中，分别有22株（19%）分离株和4株（3.5%）分离株为产EAST-1大肠杆菌和EAggEC，而在对照组（79名儿童）中，有4株（5%）分离株产生EAST-1（p = 0.005），3株（3.8%）分离株为EAggEC。本研究表明，产EAST-1大肠杆菌菌株与西班牙儿童腹泻病有关，而EAggEC菌株则无关。此外，产EAST-1大肠杆菌菌株对除氨苄西林外的所有测试抗菌药物均表现出高度敏感性。
