Jenkins C, Chart H, Willshaw G A, Cheasty T, Tompkins D S
Department of Microbiology, Royal Free Hospital, London, UK.
Eur J Clin Microbiol Infect Dis. 2007 Dec;26(12):901-6. doi: 10.1007/s10096-007-0388-z.
The aim of this study was to compare genotypic characteristics seen in typical EAggEC isolated during a study of intestinal infectious disease from cases and controls, and to identify which genes, or combinations of genes, were most associated with diarrhoeal disease. We also investigated the association of genotype with certain characteristics, such as presence of fimbrial genes and adherence to Hep-2 cells. The aafC gene, encoding the usher for AAFII, was the only gene significantly associated with patients with diarrhoea (P < 0.005), and the aggC gene, which encodes the usher for AAFI, was the only gene significantly associated with the healthy control group (P < 0.002). Putative virulence genes significantly associated with aggregative adherence included aafC, aggR, pet, pic and astA. The shf, pet and astA genes were all more likely to be associated with type II fimbriae than with type I. We conclude that in addition to presence and absence of certain genes, studies of EAggEC pathogenicity should investigate the combinations and associations of putative virulence factors.
本研究的目的是比较在一项肠道传染病研究中从病例和对照中分离出的典型肠集聚性大肠杆菌（EAggEC）的基因型特征，并确定哪些基因或基因组合与腹泻病最相关。我们还研究了基因型与某些特征的关联，如菌毛基因的存在以及对Hep-2细胞的黏附。编码AAFII usher的aafC基因是唯一与腹泻患者显著相关的基因（P < 0.005），而编码AAFI usher的aggC基因是唯一与健康对照组显著相关的基因（P < 0.002）。与集聚性黏附显著相关的假定毒力基因包括aafC、aggR、pet、pic和astA。shf、pet和astA基因与II型菌毛的关联比与I型菌毛的关联更常见。我们得出结论，除了某些基因的存在与否，对EAggEC致病性的研究还应调查假定毒力因子的组合和关联。
