Ligozzi M, Pelosi E, Fontana R
Institute of Microbiology, University of Verona, Italy.
J Med Microbiol. 1998 Oct;47(10):933-6. doi: 10.1099/00222615-47-10-933.
A competitive polymerase chain reaction (cPCR) assay for the quantitative evaluation of Mycobacterium tuberculosis growth was developed based on co-amplification of genomic DNA and a modified DNA fragment derived from a well-conserved region of the 16S rRNA gene. There was a good correlation between the number of DNA copies in the sample, indicated by competitive PCR, and the number of colony forming units determined by conventional culture methods.
