Imperiali F G, Zaninoni A, La Maestra L, Tarsia P, Blasi F, Barcellini W
Division of Hematology, University of Milan, IRCCS Ospedale Maggiore, Milan, Italy.
Clin Exp Immunol. 2001 Mar;123(3):435-42. doi: 10.1046/j.1365-2249.2001.01481.x.
Synergism between Mycobacterium tuberculosis (M. tuberculosis) and HIV-1 infections was demonstrated in several in vitro models and clinical studies. Here, we investigated their reciprocal effects on growth in chronically HIV-1-infected promonocytic U1 cells and in acutely infected monocyte-derived macrophages (MDM). Phagocytosis of M. tuberculosis induced HIV-1 expression in U1 cells, together with increased TNF-alpha production. M. tuberculosis growth, evaluated by competitive PCR, was greater in HIV-1-infected MDM compared to uninfected cells. M. tuberculosis phagocytosis induced greater TNF-alpha and IL-10 production in HIV-1-infected MDM than in uninfected cells. In uninfected MDM, addition of TNF-alpha and IFN-gamma decreased, whereas IL-10 increased M. tuberculosis growth. On the contrary, in HIV-1-infected MDM, addition of TNF-alpha and IFN-gamma increased, whereas IL-10 has no effect on M. tuberculosis growth. TNF-alpha seems to play a pivotal role in the enhanced M. tuberculosis growth observed in HIV-1-infected MDM, being unable to exert its physiological antimycobacterial activity. Here, for the first time we demonstrated an enhanced M. tuberculosis growth in HIV-1-infected MDM, in line with the observed clinical synergism between the two infections.
结核分枝杆菌（M. tuberculosis）与HIV-1感染之间的协同作用已在多个体外模型和临床研究中得到证实。在此，我们研究了它们对慢性HIV-1感染的前单核细胞U1细胞和急性感染的单核细胞衍生巨噬细胞（MDM）生长的相互影响。结核分枝杆菌的吞噬作用诱导了U1细胞中HIV-1的表达，同时TNF-α的产生增加。通过竞争性PCR评估，HIV-1感染的MDM中结核分枝杆菌的生长比未感染细胞中的更大。结核分枝杆菌的吞噬作用在HIV-1感染的MDM中比在未感染细胞中诱导产生更多的TNF-α和IL-10。在未感染的MDM中，添加TNF-α和IFN-γ会降低结核分枝杆菌的生长，而IL-10则会增加其生长。相反，在HIV-1感染的MDM中，添加TNF-α和IFN-γ会增加结核分枝杆菌的生长，而IL-10对其生长没有影响。TNF-α似乎在HIV-1感染的MDM中观察到的结核分枝杆菌生长增强中起关键作用，无法发挥其生理抗分枝杆菌活性。在此，我们首次证明了HIV-1感染的MDM中结核分枝杆菌生长增强，这与两种感染之间观察到的临床协同作用一致。
