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1
Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex.与非天然底物的酶促反应：DNA光解酶（大肠杆菌）识别并逆转DNA/PNA杂交双链体DNA链中的胸腺嘧啶[2+2]二聚体。
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12902-5. doi: 10.1073/pnas.95.22.12902.
2
DNA photolyase repairs the trans-syn cyclobutane thymine dimer.DNA光解酶修复反式-顺式环丁烷胸腺嘧啶二聚体。
Biochemistry. 1993 Jul 20;32(28):7065-8. doi: 10.1021/bi00079a001.
3
Identification of oligothymidylates as new simple substrates for Escherichia coli DNA photolyase and their use in a rapid spectrophotometric enzyme assay.鉴定寡聚胸苷酸作为大肠杆菌DNA光解酶的新型简单底物及其在快速分光光度酶测定中的应用。
Biochemistry. 1985 Apr 9;24(8):1856-61. doi: 10.1021/bi00329a008.
4
Effect of base, pentose, and phosphodiester backbone structures on binding and repair of pyrimidine dimers by Escherichia coli DNA photolyase.碱基、戊糖和磷酸二酯主链结构对大肠杆菌DNA光解酶结合和修复嘧啶二聚体的影响。
Biochemistry. 1991 Sep 3;30(35):8623-30. doi: 10.1021/bi00099a019.
5
Enzyme-Substrate Binding Kinetics Indicate That Photolyase Recognizes an Extrahelical Cyclobutane Thymidine Dimer.酶-底物结合动力学表明光解酶识别螺旋外的环丁烷胸腺嘧啶二聚体。
Biochemistry. 2015 Oct 13;54(40):6176-85. doi: 10.1021/acs.biochem.5b00927. Epub 2015 Oct 1.
6
Effect of sequence, adduct type, and opposing lesions on the binding and repair of ultraviolet photodamage by DNA photolyase and (A)BC excinuclease.序列、加合物类型及相对损伤对DNA光解酶和（A）BC核酸外切酶结合与修复紫外线光损伤的影响。
J Biol Chem. 1993 May 15;268(14):10694-700.
7
Photorepair of nonadjacent pyrimidine dimers by DNA photolyase.DNA光解酶对非相邻嘧啶二聚体的光修复作用。
Photochem Photobiol. 1995 Feb;61(2):171-4. doi: 10.1111/j.1751-1097.1995.tb03956.x.
8
Substrate range of the 40,000-dalton DNA-photoreactivating enzyme from Escherichia coli.来自大肠杆菌的40000道尔顿DNA光复活酶的底物范围。
Biochemistry. 1986 Feb 11;25(3):681-7. doi: 10.1021/bi00351a026.
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The photo repair of pyrimidine dimers by DNA photolyase and model systems.DNA光解酶和模型系统对嘧啶二聚体的光修复
J Photochem Photobiol B. 1993 Mar;17(3):219-28. doi: 10.1016/1011-1344(93)80019-6.
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Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair.原位修复后与类CPD DNA损伤结合的光解酶的晶体结构。
Science. 2004 Dec 3;306(5702):1789-93. doi: 10.1126/science.1101598.

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Electrically monitoring DNA repair by photolyase.通过光裂合酶对DNA修复进行电学监测。
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本文引用的文献

1
The selective degradation of pyrimidines in nucleic acids by permanganate oxidation.通过高锰酸盐氧化对核酸中嘧啶进行选择性降解。
Biochem Biophys Res Commun. 1967 Nov 30;29(4):556-61. doi: 10.1016/0006-291x(67)90521-9.
2
Are polyphosphates or phosphate esters prebiotic reagents?多磷酸盐或磷酸酯是益生元试剂吗？
J Mol Evol. 1995 Dec;41(6):693-702. doi: 10.1007/BF00173147.
3
Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.DNA中胸腺嘧啶[2+2]光二聚体修复的检测：高锰酸钾的选择性反应
Nucleic Acids Res. 1998 Sep 1;26(17):3940-3. doi: 10.1093/nar/26.17.3940.
4
Synthesis and evaluation of some properties of chimeric oligomers containing PNA and phosphono-PNA residues.含肽核酸（PNA）和膦酰基 - 肽核酸残基的嵌合寡聚物的合成及某些性质的评估
Nucleic Acids Res. 1998 Jan 15;26(2):566-75. doi: 10.1093/nar/26.2.566.
5
Peptide nucleic acid-DNA duplexes: long range hole migration from an internally linked anthraquinone.肽核酸 - DNA 双链体：来自内部连接蒽醌的长程空穴迁移
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12320-5. doi: 10.1073/pnas.94.23.12320.
6
DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate.阳离子锰卟啉-肽核酸共轭物的DNA结合与切割
Bioconjug Chem. 1997 May-Jun;8(3):267-70. doi: 10.1021/bc9700190.
7
Solution structure of a peptide nucleic acid-DNA duplex.肽核酸 - DNA 双链体的溶液结构
Nat Struct Biol. 1996 May;3(5):410-3. doi: 10.1038/nsb0596-410.
8
Structure and function of DNA photolyase.DNA光解酶的结构与功能。
Biochemistry. 1994 Jan 11;33(1):2-9. doi: 10.1021/bi00167a001.
9
Stability of peptide nucleic acids in human serum and cellular extracts.肽核酸在人血清和细胞提取物中的稳定性。
Biochem Pharmacol. 1994 Sep 15;48(6):1310-3. doi: 10.1016/0006-2952(94)90171-6.
10
Interactions of DNA binding ligands with PNA-DNA hybrids.DNA结合配体与肽核酸- DNA杂交体的相互作用。
Nucleic Acids Res. 1994 Dec 11;22(24):5371-7. doi: 10.1093/nar/22.24.5371.

与非天然底物的酶促反应：DNA光解酶（大肠杆菌）识别并逆转DNA/PNA杂交双链体DNA链中的胸腺嘧啶[2+2]二聚体。

Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex.

作者信息

Ramaiah D, Kan Y, Koch T, Orum H, Schuster G B

机构信息

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

出版信息

Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12902-5. doi: 10.1073/pnas.95.22.12902.

DOI:10.1073/pnas.95.22.12902
PMID:9789012
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC23647/
Abstract

Peptide nucleic acids (PNA) are mimics with normal bases connected to a pseudopeptide chain that obey Watson-Crick rules to form stable duplexes with itself and natural nucleic acids. This has focused attention on PNA as therapeutic or diagnostic reagents. Duplexes formed with PNA mirror some but not all properties of DNA. One fascinating aspect of PNA biochemistry is their reaction with enzymes. Here we show an enzyme reaction that operates effectively on a PNA/DNA hybrid duplex. A DNA oligonucleotide containing a cis, syn-thymine [2+2] dimer forms a stable duplex with PNA. The hybrid duplex is recognized by photolyase, and irradiation of the complex leads to the repair of the thymine dimer. This finding provides insight into the enzyme mechanism and provides a means for the selective repair of thymine photodimers.

摘要

肽核酸（PNA）是一种模拟物，其正常碱基连接到一条假肽链上，该假肽链遵循沃森-克里克规则，可与自身以及天然核酸形成稳定的双链体。这使得人们将注意力集中在PNA作为治疗或诊断试剂上。与PNA形成的双链体反映了DNA的一些但并非全部特性。PNA生物化学的一个迷人之处在于它们与酶的反应。在这里，我们展示了一种在PNA/DNA杂交双链体上有效起作用的酶反应。一种含有顺式、同向胸腺嘧啶[2+2]二聚体的DNA寡核苷酸与PNA形成稳定的双链体。这种杂交双链体被光解酶识别，对该复合物进行照射会导致胸腺嘧啶二聚体的修复。这一发现为酶的作用机制提供了见解，并为胸腺嘧啶光二聚体的选择性修复提供了一种方法。