• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.DNA中胸腺嘧啶[2+2]光二聚体修复的检测：高锰酸钾的选择性反应
Nucleic Acids Res. 1998 Sep 1;26(17):3940-3. doi: 10.1093/nar/26.17.3940.
2
Photoreactivation of the thymine dimer containing DNA octamer d(GCGTTGCG).d(CGCAACGC) by the photoreactivating enzyme from Anacystis nidulans.来自集胞藻的光复活酶对含有胸腺嘧啶二聚体的DNA八聚体d(GCGTTGCG).d(CGCAACGC)的光复活作用
J Photochem Photobiol B. 1988 Mar;1(3):323-8. doi: 10.1016/1011-1344(88)85020-6.
3
Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex.与非天然底物的酶促反应：DNA光解酶（大肠杆菌）识别并逆转DNA/PNA杂交双链体DNA链中的胸腺嘧啶[2+2]二聚体。
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12902-5. doi: 10.1073/pnas.95.22.12902.
4
Recent aspects of the photochemistry of nucleic acids and related model compounds.核酸及相关模型化合物光化学的近期研究进展。
Biochimie. 1985 Mar-Apr;67(3-4):277-92. doi: 10.1016/s0300-9084(85)80070-5.
5
Spore photoproduct (SP) lyase from Bacillus subtilis specifically binds to and cleaves SP (5-thyminyl-5,6-dihydrothymine) but not cyclobutane pyrimidine dimers in UV-irradiated DNA.来自枯草芽孢杆菌的孢子光产物（SP）裂合酶特异性结合并切割紫外线照射的DNA中的SP（5-胸腺嘧啶基-5,6-二氢胸腺嘧啶），但不切割环丁烷嘧啶二聚体。
J Bacteriol. 2000 Nov;182(22):6412-7. doi: 10.1128/JB.182.22.6412-6417.2000.
6
[Photochemical reactions and photoreactivation of DNA].[DNA的光化学反应与光复活作用]
Biochimie. 1978;60(10):1111-21.
7
A comparative repair study of thymine- and uracil-photodimers with model compounds and a photolyase repair enzyme.胸腺嘧啶和尿嘧啶光二聚体与模型化合物及光解酶修复酶的比较修复研究
Chemistry. 2000 Jan;6(1):62-72. doi: 10.1002/(sici)1521-3765(20000103)6:1<62::aid-chem62>3.0.co;2-7.
8
Mapping cyclobutane-pyrimidine dimers in DNA and using DNA-repair by photolyase for chromatin analysis in yeast.绘制DNA中的环丁烷嘧啶二聚体，并利用光解酶进行DNA修复以分析酵母中的染色质。
Methods Enzymol. 1999;304:447-61. doi: 10.1016/s0076-6879(99)04027-6.
9
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair.原位修复后与类CPD DNA损伤结合的光解酶的晶体结构。
Science. 2004 Dec 3;306(5702):1789-93. doi: 10.1126/science.1101598.
10
Identification of oligothymidylates as new simple substrates for Escherichia coli DNA photolyase and their use in a rapid spectrophotometric enzyme assay.鉴定寡聚胸苷酸作为大肠杆菌DNA光解酶的新型简单底物及其在快速分光光度酶测定中的应用。
Biochemistry. 1985 Apr 9;24(8):1856-61. doi: 10.1021/bi00329a008.

引用本文的文献

1
Thymine dimer-induced structural changes to the DNA duplex examined with reactive probes (†).用反应性探针检测胸腺嘧啶二聚体诱导的DNA双链结构变化(†)。
Biochemistry. 2008 Dec 9;47(49):13026-35. doi: 10.1021/bi801417u.
2
Substrate binding to DNA photolyase studied by electron paramagnetic resonance spectroscopy.通过电子顺磁共振光谱研究底物与DNA光解酶的结合
Biophys J. 2001 Aug;81(2):1195-204. doi: 10.1016/S0006-3495(01)75777-3.
3
Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex.与非天然底物的酶促反应：DNA光解酶（大肠杆菌）识别并逆转DNA/PNA杂交双链体DNA链中的胸腺嘧啶[2+2]二聚体。
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12902-5. doi: 10.1073/pnas.95.22.12902.

本文引用的文献

1
The selective degradation of pyrimidines in nucleic acids by permanganate oxidation.通过高锰酸盐氧化对核酸中嘧啶进行选择性降解。
Biochem Biophys Res Commun. 1967 Nov 30;29(4):556-61. doi: 10.1016/0006-291x(67)90521-9.
2
Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast.用来自面包酵母的光复活酶处理后，紫外线照射的DNA中胸腺嘧啶光二聚体的消失
Biochem Biophys Res Commun. 1962 Apr 20;7:237-40. doi: 10.1016/0006-291x(62)90181-x.
3
Peptide nucleic acid-anthraquinone conjugates: strand invasion and photoinduced cleavage of duplex DNA.肽核酸 - 蒽醌共轭物：双链DNA的链侵入和光诱导切割
Nucleic Acids Res. 1997 Nov 15;25(22):4674-8. doi: 10.1093/nar/25.22.4674.
4
Far-UV-induced dimeric photoproducts in short oligonucleotides: sequence effects.短寡核苷酸中远紫外诱导的二聚体光产物：序列效应
Photochem Photobiol. 1997 Aug;66(2):171-9. doi: 10.1111/j.1751-1097.1997.tb08639.x.
5
Oxidative thymine dimer repair in the DNA helix.DNA螺旋中的氧化性胸腺嘧啶二聚体修复
Science. 1997 Mar 7;275(5305):1465-8. doi: 10.1126/science.275.5305.1465.
6
Structure and function of DNA photolyase.DNA光解酶的结构与功能。
Biochemistry. 1994 Jan 11;33(1):2-9. doi: 10.1021/bi00167a001.
7
Pyrimidine-specific chemical reactions useful for DNA sequencing.用于DNA测序的嘧啶特异性化学反应。
Nucleic Acids Res. 1980 Oct 24;8(20):4613-9. doi: 10.1093/nar/8.20.4613.
8
Osmium tetroxide: a new probe for site-specific distortions in supercoiled DNAs.四氧化锇：超螺旋DNA中位点特异性扭曲的新型探针。
Nucleic Acids Res. 1984 Feb 10;12(3):1725-35. doi: 10.1093/nar/12.3.1725.
9
Photosensitized splitting of pyrimidine dimers.嘧啶二聚体的光敏裂解
Photochem Photobiol. 1970 Mar;11(3):163-8. doi: 10.1111/j.1751-1097.1970.tb05984.x.
10
Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution.DNA构象的化学探针：核苷酸分辨率下Z-DNA的检测
Cell. 1985 Oct;42(3):713-24. doi: 10.1016/0092-8674(85)90268-5.

DNA中胸腺嘧啶[2+2]光二聚体修复的检测：高锰酸钾的选择性反应

Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.

作者信息

Ramaiah D, Koch T, Orum H, Schuster G B

机构信息

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

出版信息

Nucleic Acids Res. 1998 Sep 1;26(17):3940-3. doi: 10.1093/nar/26.17.3940.

DOI:10.1093/nar/26.17.3940
PMID:9705501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147792/
Abstract

The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.

摘要

利用高锰酸钾与单链DNA中胸腺嘧啶的特异性反应，分析DNA及DNA/肽核酸杂交双链中胸腺嘧啶[2+2]二聚体的修复情况。这种简单且高度灵敏的化学分析方法便于监测寡核苷酸中胸腺嘧啶二聚体的修复过程。