DNA中胸腺嘧啶[2+2]光二聚体修复的检测：高锰酸钾的选择性反应
Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
作者信息
Ramaiah D, Koch T, Orum H, Schuster G B
机构信息
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
出版信息
Nucleic Acids Res. 1998 Sep 1;26(17):3940-3. doi: 10.1093/nar/26.17.3940.
Abstract
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
摘要
利用高锰酸钾与单链DNA中胸腺嘧啶的特异性反应，分析DNA及DNA/肽核酸杂交双链中胸腺嘧啶[2+2]二聚体的修复情况。这种简单且高度灵敏的化学分析方法便于监测寡核苷酸中胸腺嘧啶二聚体的修复过程。
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本文引用的文献
1
The selective degradation of pyrimidines in nucleic acids by permanganate oxidation.
Biochem Biophys Res Commun. 1967 Nov 30;29(4):556-61. doi: 10.1016/0006-291x(67)90521-9.
2
Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast.
Biochem Biophys Res Commun. 1962 Apr 20;7:237-40. doi: 10.1016/0006-291x(62)90181-x.
3
Peptide nucleic acid-anthraquinone conjugates: strand invasion and photoinduced cleavage of duplex DNA.
Nucleic Acids Res. 1997 Nov 15;25(22):4674-8. doi: 10.1093/nar/25.22.4674.
4
Far-UV-induced dimeric photoproducts in short oligonucleotides: sequence effects.
Photochem Photobiol. 1997 Aug;66(2):171-9. doi: 10.1111/j.1751-1097.1997.tb08639.x.
5
Oxidative thymine dimer repair in the DNA helix.
Science. 1997 Mar 7;275(5305):1465-8. doi: 10.1126/science.275.5305.1465.
6
Structure and function of DNA photolyase.
Biochemistry. 1994 Jan 11;33(1):2-9. doi: 10.1021/bi00167a001.
7
Pyrimidine-specific chemical reactions useful for DNA sequencing.
Nucleic Acids Res. 1980 Oct 24;8(20):4613-9. doi: 10.1093/nar/8.20.4613.
8
Osmium tetroxide: a new probe for site-specific distortions in supercoiled DNAs.
Nucleic Acids Res. 1984 Feb 10;12(3):1725-35. doi: 10.1093/nar/12.3.1725.
9
Photosensitized splitting of pyrimidine dimers.
Photochem Photobiol. 1970 Mar;11(3):163-8. doi: 10.1111/j.1751-1097.1970.tb05984.x.
10
Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution.
Cell. 1985 Oct;42(3):713-24. doi: 10.1016/0092-8674(85)90268-5.
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