DNA中胸腺嘧啶[2+2]光二聚体修复的检测：高锰酸钾的选择性反应
Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
作者信息
Ramaiah D, Koch T, Orum H, Schuster G B
机构信息
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
出版信息
Nucleic Acids Res. 1998 Sep 1;26(17):3940-3. doi: 10.1093/nar/26.17.3940.
Abstract
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
摘要
利用高锰酸钾与单链DNA中胸腺嘧啶的特异性反应，分析DNA及DNA/肽核酸杂交双链中胸腺嘧啶[2+2]二聚体的修复情况。这种简单且高度灵敏的化学分析方法便于监测寡核苷酸中胸腺嘧啶二聚体的修复过程。
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