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细胞凋亡检测:概述

Apoptosis detection: an overview.

作者信息

Sgonc R, Gruber J

机构信息

Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Austria.

出版信息

Exp Gerontol. 1998 Sep;33(6):525-33. doi: 10.1016/s0531-5565(98)00031-x.

DOI:10.1016/s0531-5565(98)00031-x
PMID:9789730
Abstract

As the analysis of apoptosis is of interest in many basic and clinically oriented investigations, we intend to give a brief overview on the recently most-used methods for detection of apoptotic cells, including the study of morphology, analysis of DNA degradation, DNA end-labeling techniques, flow cytometric analysis, and nuclease assays. Features and advantages of the different methods are discussed.

摘要

由于细胞凋亡分析在许多基础研究和临床研究中都备受关注,我们打算简要概述一下近年来最常用的检测凋亡细胞的方法,包括形态学研究、DNA降解分析、DNA末端标记技术、流式细胞术分析和核酸酶检测。文中还讨论了不同方法的特点和优势。

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