Bustos P, Calvo C, Ulloa N, Quiroga A, Sepulveda J
Departamento Bioquímica Clínica e Inmunología, Facultad de Farmacia, Universidad de Concepción, Chile.
Hybridoma. 1998 Aug;17(4):395-401. doi: 10.1089/hyb.1998.17.395.
The present report describes a new monoclonal antibody-based enzyme immunoassay (ELISA) for the quantification of apolipoprotein (apo) B-containing particles in plasma. Native low-density lipoprotein (LDL) and a reference serum were utilized to prepare the standard curve. Three different antibodies to apo B-100--4A6E3, 6A10B10, and 2D9--all produced in our laboratory, were examined. The apparent affinity constants of the monoclonal antibodies (MAbs) 4A6E3, 6A10B10, and 2D9 were 2.9 x 10(9), 1.74 x 10(9), and 0.63 x 10(8), respectively. The standard curve was generated for an apo B-LDL range of 0.1 to 4.0 microg/ml. Evaluating the concentration of apo B-containing particles in plasma may allow for a more accurate assessment of the risk of coronary artery disease.
本报告描述了一种基于单克隆抗体的酶免疫测定法（ELISA），用于定量血浆中含载脂蛋白（apo）B的颗粒。利用天然低密度脂蛋白（LDL）和参考血清制备标准曲线。检测了三种针对apo B-100的不同抗体——4A6E3、6A10B10和2D9——均在我们实验室生产。单克隆抗体（MAb）4A6E3、6A10B10和2D9的表观亲和常数分别为2.9×10⁹、1.74×10⁹和0.63×10⁸。生成了apo B-LDL范围为0.1至4.0微克/毫升的标准曲线。评估血浆中含apo B颗粒的浓度可能有助于更准确地评估冠状动脉疾病的风险。
