Fushimi F, Nakayama M, Nishimura K, Hiyoshi T
Hollow Fiber Plant, Asahi Chemical Industry Co., Ltd., Nobeoka, Miyazaki, Japan.
Artif Organs. 1998 Oct;22(10):821-6. doi: 10.1046/j.1525-1594.1998.06046.x.
Grafting of polyethylene glycol chains onto cellulosic membrane can be expected to reduce the interaction between blood (plasma protein and cells) and the membrane surface. Alkylether carboxylic acid (PEG acid) grafted high flux cellulosic membranes for hemodialysis, in which the polyethylene glycol chain bears an alkyl group at one side and a carboxyl group at the other side, have been developed and evaluated. PEG acid-grafted high flux cellulosic membranes with various grafting amounts have been compared with respect to platelet adhesion, the contact phase of blood coagulation, and complement activation in vitro. A new method of quantitating platelet adhesion on hollow-fiber membrane surfaces has been developed, which is based on the determination of lactate dehydrogenase (LDH) activity after lysis of the adhered platelets. PEG acid-grafted high flux cellulosic membranes showed reduced platelet adhesion and complement activation effects in grafting amounts of 200 ppm or higher without detecting adverse effects up to grafting amounts of 850 ppm. The platelet adhesion of a PEG acid-grafted cellulosic membrane depends on both the flux and grafting amounts of the membrane. It is concluded that the grafting of PEG acid onto a cellulosic membrane improves its biocompatibility as evaluated in terms of platelet adhesion, complement activation, and thrombogenicity.
将聚乙二醇链接枝到纤维素膜上有望减少血液（血浆蛋白和细胞）与膜表面之间的相互作用。已经开发并评估了用于血液透析的烷基醚羧酸（PEG酸）接枝的高通量纤维素膜，其中聚乙二醇链一侧带有烷基，另一侧带有羧基。比较了不同接枝量的PEG酸接枝高通量纤维素膜在血小板黏附、血液凝固接触相和体外补体激活方面的情况。开发了一种定量中空纤维膜表面血小板黏附的新方法，该方法基于测定黏附血小板裂解后的乳酸脱氢酶（LDH）活性。PEG酸接枝的高通量纤维素膜在接枝量为200 ppm或更高时显示出降低的血小板黏附和补体激活作用，在接枝量高达850 ppm时未检测到不良反应。PEG酸接枝纤维素膜的血小板黏附取决于膜的通量和接枝量。结论是，从血小板黏附、补体激活和血栓形成性方面评估，将PEG酸接枝到纤维素膜上可改善其生物相容性。
