Zhang Y, LeRoy G, Seelig H P, Lane W S, Reinberg D
Howard Hughes Medical Institute, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
Cell. 1998 Oct 16;95(2):279-89. doi: 10.1016/s0092-8674(00)81758-4.
Histone acetylation and deacetylation were found to be catalyzed by structurally distinct, multisubunit complexes that mediate, respectively, activation and repression of transcription. ATP-dependent nucleosome remodeling, mediated by different multisubunit complexes, was thought to be involved only in transcription activation. Here we report the isolation of a protein complex that contains both histone deacetylation and ATP-dependent nucleosome remodeling activities. The complex contains the histone deacetylases HDAC1/2, histone-binding proteins, the dermatomyositis-specific autoantigen Mi2beta, a polypeptide related to the metastasis-associated protein 1, and a novel polypeptide of 32 kDa. Patients with dermatomyositis have a high rate of malignancy. The finding that Mi2beta exists in a complex containing histone deacetylase and nucleosome remodeling activities suggests a role for chromatin reorganization in cancer metastasis.
人们发现，组蛋白乙酰化和去乙酰化分别由结构不同的多亚基复合物催化，这些复合物分别介导转录的激活和抑制。由不同多亚基复合物介导的ATP依赖型核小体重塑，曾被认为仅参与转录激活。在此我们报告分离出一种包含组蛋白去乙酰化和ATP依赖型核小体重塑活性的蛋白质复合物。该复合物含有组蛋白去乙酰化酶HDAC1/2、组蛋白结合蛋白、皮肌炎特异性自身抗原Mi2β、一种与转移相关蛋白1相关的多肽以及一种32 kDa的新型多肽。皮肌炎患者的恶性肿瘤发生率很高。Mi2β存在于一个包含组蛋白去乙酰化酶和核小体重塑活性的复合物中的这一发现，提示染色质重组在癌症转移中发挥作用。
