Buckmire M A, Parquet G, Greenway S, Rolandelli R H
Philadelphia Veterans Administration Medical Center, Medical College of Pennsylvania/Hahnemann University, Philadelphia, Pennsylvania, 19102-1192, USA.
J Surg Res. 1998 Nov;80(1):52-7. doi: 10.1006/jsre.1998.5326.
Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF-beta1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF-beta1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene.
Forty-eight Sprague-Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on Day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-beta1, EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene.
The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-beta1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 +/- 0. 08 on Day 0 to a mean ratio of 1.9 +/- 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-beta1 gene (r = 0.89, P < 0.05).
The temporal correlation between an increase in the gene expression of TGF-beta1 and PROC I is initial evidence that that TGF-beta1 plays a significant role in collagen metabolism in a healing colonic anastomosis.
结肠吻合口裂开是一种多因素现象。其发生的一种机制是结肠黏膜下胶原蛋白缺乏。肽生长因子（PGFs）已被证明在胶原蛋白的合成、沉积和成熟过程中起作用。具体而言，在结肠以外的组织中，转化生长因子-β（TGF-β1）基因已被证明与前胶原基因的表达在时间上相关。本研究检测了TGF-β1、表皮生长因子（EGF）和血小板衍生生长因子B（PDGF-BB）基因的时间表达情况以及它们与I型前胶原（PROC I）基因表达的时间关系。
48只Sprague-Dawley大鼠接受降结肠横断并进行一期吻合。在术后第0天以及术后第3、5、6、7和14天采集吻合口周围的结肠组织。使用半定量逆转录聚合酶链反应和针对TGF-β1、EGF和PDGF-B生长因子的特异性引物对结肠组织进行分析。计算PGFs和PROC I基因相对于组成型基因的相对表达率。
数据显示，虽然所有三种PGFs基因均在术后愈合的结肠组织中表达，但与组成型基因相比，只有TGF-β1的表达水平显著增加，从第0天的平均比值0.4±0.08增加到第7天的平均比值1.9±0.27（方差分析，P<0.0001）。PROC I基因在术后也显示出表达显著增加（方差分析，P<0.001），且在时间上与TGF-β1基因表达的增加相关（r = 0.89，P<0.05）。
TGF-β1基因表达增加与PROC I之间的时间相关性初步证明，TGF-β1在愈合的结肠吻合口中的胶原蛋白代谢中起重要作用。
