Spatz A M, Ali S A, Auer M, Graf C, Eibl M M, Steinkasserer A
Institut für Bakteriologie, Mykologie, und Hygiene, Veterinärmedizinische Universität Wien, Veterinaerplatz 1, Vienna, A-1210, Austria.
Biochem Biophys Res Commun. 1998 Oct 9;251(1):235-8. doi: 10.1006/bbrc.1998.9447.
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % alpha-helix and 17.5 % beta-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies.
