Hippenmeyer P J, Pegg L E
GD Searle, St Louis, USA.
Curr Opin Biotechnol. 1995 Oct;6(5):548-52. doi: 10.1016/0958-1669(95)80091-3.
The herpesvirus VP16 transactivator has become a useful tool for facilitating the production of recombinant proteins in cultured mammalian cells. Not only does it afford the rapid isolation of stable high-level producer cell lines, but also VP16-expressing cells have been found to rival COS cells in their ability to express proteins transiently. Some of the most interesting developments have been the expression of heterodimeric receptors and soluble forms of membrane proteins.
疱疹病毒VP16反式激活因子已成为促进在培养的哺乳动物细胞中生产重组蛋白的一种有用工具。它不仅能快速分离出稳定的高表达细胞系，而且还发现表达VP16的细胞在瞬时表达蛋白的能力上可与COS细胞相媲美。一些最有趣的进展是异二聚体受体和膜蛋白可溶性形式的表达。
