Novak C M, Nunez A A
Department of Psychology and Neuroscience Program, Michigan State University, East Lansing, Michigan 48824-1117, USA.
Am J Physiol. 1998 Nov;275(5):R1620-6. doi: 10.1152/ajpregu.1998.275.5.R1620.
The present experiment investigated the expression of the nuclear phosphoprotein Fos over the 24-h light-dark cycle in regions of the rat brain related to sleep and vigilance, including the ventrolateral preoptic area (VLPO), the paraventricular thalamic nucleus (PVT), and the central medial thalamic nucleus (CMT). Immunocytochemistry for Fos, an immediate-early gene product used as an index of neuronal activity, was carried out on brain sections from rats perfused at zeitgeber time (ZT) 1, ZT 5, ZT 12.5, and ZT 17 (lights on ZT 0-ZT 12). The number of Fos-immunopositive (Fos+) cells in the VLPO was elevated at ZT 5 and 12.5 (i.e., during or just after the rest phase of the cycle). Fos+ cell number increased at ZT 17 and ZT 1 in the PVT and CMT, 180 degrees out of phase with the VLPO. A positive correlation was found between the numbers of Fos+ cells in the PVT and CMT, and Fos expression in each thalamic nucleus was negatively correlated with VLPO Fos+ cell number. The VLPO, PVT, and CMT may integrate circadian and homeostatic influences to regulate the sleep-wake cycle.
本实验研究了核磷蛋白Fos在大鼠大脑中与睡眠和警觉相关区域（包括腹外侧视前区（VLPO）、室旁丘脑核（PVT）和丘脑中央内侧核（CMT））的24小时明暗周期中的表达情况。Fos是一种即时早期基因产物，用作神经元活动的指标，对在授时因子时间（ZT）1、ZT 5、ZT 12.5和ZT 17（ZT 0-ZT 12为光照时间）处死的大鼠脑切片进行Fos免疫细胞化学检测。VLPO中Fos免疫阳性（Fos+）细胞的数量在ZT 5和12.5时升高（即在周期的休息阶段期间或之后）。PVT和CMT中Fos+细胞数量在ZT 17和ZT 1时增加，与VLPO相差180度。PVT和CMT中Fos+细胞数量之间存在正相关，每个丘脑核中的Fos表达与VLPO Fos+细胞数量呈负相关。VLPO、PVT和CMT可能整合昼夜节律和稳态影响以调节睡眠-觉醒周期。
