Pérez J A, Rodríguez J, Rodríguez L, Ruiz T
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, E-38071 La Laguna, Tenerife, Spain.
Curr Genet. 1996 Feb;29(3):234-40. doi: 10.1007/BF02221553.
A genomic library from the yeast Pichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant of Saccharomyces cerevisiae. The cloned gene, INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also with Kluyveromyces marxianus inulinase, a yeast beta-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.
已构建了异常毕赤酵母的基因组文库，并通过互补酿酒酵母的蔗糖非发酵突变体来克隆编码蔗糖水解酶转化酶的基因。对克隆的基因INV1进行了测序，发现它编码一个由550个氨基酸组成的多肽，该多肽包含一个22个氨基酸的信号序列和十个潜在的糖基化位点。氨基酸序列与其他酵母转化酶以及马克斯克鲁维酵母菊粉酶具有显著的同源性，马克斯克鲁维酵母菊粉酶是一种具有不同底物特异性的酵母β-呋喃果糖苷酶。发现5'和3'非编码区的核苷酸序列包含几个可能参与基因转录起始和终止的共有基序。
