Ikemoto K, Nagatsu I, Ito S, King R A, Nishimura A, Nagatsu T
Department of Anatomy, Fujita Health University, School of Medicine, Toyoake, Japan.
Neurosci Lett. 1998 Sep 11;253(3):198-200. doi: 10.1016/s0304-3940(98)00649-1.
It is controversial whether tyrosinase is involved in the neuromelanin-biosynthetic pathway. We examined tyrosinase-immunoreactivity in human substantia nigra neurons which contain neuromelanin pigments, using antibodies against human tyrosinase and human tyrosine hydroxylase. In human melanoma, the antibody to tyrosinase showed intense immunoreactivity while there was no immunoreactivity with antibody to tyrosine hydroxylase. In the human midbrain pigmented neurons, however, we could detect no tyrosinase-immunoreactivity while the neurons were strongly immunoreactive to tyrosine hydroxylase. The present results suggest that tyrosinase is not involved in the main pathway of neuromelanin biosynthesis.
酪氨酸酶是否参与神经黑色素的生物合成途径存在争议。我们使用抗人酪氨酸酶和抗人酪氨酸羟化酶的抗体，检测了含有神经黑色素的人黑质神经元中的酪氨酸酶免疫反应性。在人类黑色素瘤中，酪氨酸酶抗体显示出强烈的免疫反应性，而酪氨酸羟化酶抗体则无免疫反应性。然而，在人类中脑色素神经元中，我们未检测到酪氨酸酶免疫反应性，而这些神经元对酪氨酸羟化酶有强烈的免疫反应性。目前的结果表明，酪氨酸酶不参与神经黑色素生物合成的主要途径。
