刺酸对肿瘤坏死因子-α基因表达和触珠蛋白合成的影响。
Effects of acanthoic acid on TNF-alpha gene expression and haptoglobin synthesis.
作者信息
Kang H S, Song H K, Lee J J, Pyun K H, Choi I
机构信息
Immune Cell Signal Transduction Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Republic of Korea.
出版信息
Mediators Inflamm. 1998;7(4):257-9. doi: 10.1080/09629359890938.
Tumour necrosis factor-alpha (TNF-alpha) is a major pro-inflammatory cytokine inducing the synthesis and release of many inflammatory mediators. It is involved in immune regulation, autoimmune diseases, and inflammation. Our previous study demonstrated that acanthoic acid, (-)-pimara-9(11), 15-dien-19-oic acid, a pimaradiene diterpene isolated from Acanthopanax koreanum, inhibited TNF-alpha production. To extend our understanding of inhibitory effects of acanthoic acid on TNF-alpha production, its effects on TNF-alpha gene expression was tested. Based on the results from RT-PCR and promoter analysis of TNF-alpha, it was found that acanthoic acid suppressed TNF-alpha gene expression. But the same concentration of acanthoic acid had no effect on IL-6 gene expression. Haptoglobin is an acute phase protein which is induced by TNF-alpha. When liver cells were treated with acanthoic acid, haptoglobin synthesis was blocked by acanthoic acid. These data confirmed that acanthoic acid inhibited gene expression and biological function of TNF-alpha.
肿瘤坏死因子-α（TNF-α）是一种主要的促炎细胞因子，可诱导多种炎症介质的合成与释放。它参与免疫调节、自身免疫性疾病和炎症过程。我们之前的研究表明，从刺五加中分离出的一种半日花二萜类化合物刺五加酸（(-)-海松-9(11),15-二烯-19-酸）可抑制TNF-α的产生。为了进一步了解刺五加酸对TNF-α产生的抑制作用，我们测试了其对TNF-α基因表达的影响。基于TNF-α的逆转录-聚合酶链反应（RT-PCR）和启动子分析结果，发现刺五加酸可抑制TNF-α基因表达。但相同浓度的刺五加酸对白细胞介素-6（IL-6）基因表达没有影响。触珠蛋白是一种由TNF-α诱导产生的急性期蛋白。当用刺五加酸处理肝细胞时，触珠蛋白的合成被刺五加酸阻断。这些数据证实，刺五加酸可抑制TNF-α的基因表达和生物学功能。
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