大鼠血浆中（±）-乙丙嗪及其对映体的高效液相色谱测定法。

High-performance liquid chromatographic assay of (+/-)-ethopropazine and its enantiomers in rat plasma.

作者信息

Maboudian-Esfahani M, Brocks D R

机构信息

College of Pharmacy and Nutrition, University of Saskatchewan, SK, Canada.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Sep 18;715(2):417-23. doi: 10.1016/s0378-4347(98)00245-x.

DOI:10.1016/s0378-4347(98)00245-x

PMID:9792528

Abstract

Two high-performance liquid chromatographic (HPLC) methods are described for determination of (+/-)-ethopropazine (ET) in rat plasma. After deproteination and liquid-liquid extraction, assay of (+/-)-ET was performed using either a C18 column (non-stereospecific assay) or an (alpha-R-naphthyl)ethylurea column (stereospecific assay). The UV detection was at 250 nm. Mean recovery was >85%. Both assays demonstrated excellent linear relationships between peak height ratios and plasma concentrations; quantitation limits were < or =25 ng/ml, based on 100 microl rat plasma. Accuracy and precision were <17% with both methods. Both methods were applied successfully to the measurement of ET plasma concentrations in rats given the drug intravenously.

摘要

描述了两种用于测定大鼠血浆中（±）-乙磺半胱氨酸（ET）的高效液相色谱（HPLC）方法。经过去蛋白和液-液萃取后，使用C18柱（非立体特异性测定法）或（α-R-萘基）乙基脲柱（立体特异性测定法）对（±）-ET进行测定。紫外检测波长为250nm。平均回收率>85%。两种测定法均显示峰高比与血浆浓度之间具有良好的线性关系；基于100μl大鼠血浆，定量限≤25ng/ml。两种方法的准确度和精密度均<17%。两种方法均成功应用于静脉注射该药物的大鼠血浆中ET浓度的测定。