Tsuchida T, Yamada A, Yoshimura K, Kawamoto K
Department of Neurosurgery, Kansai Medical School, Osaka, Japan.
Ultrastruct Pathol. 1998 May-Jun;22(3):233-8. doi: 10.3109/01913129809033474.
Central neurocytoma is a rare brain tumor with neuronal differentiation. Cultured central neurocytoma cells are poorly described because of the tumor's scarcity. Two central neurocytomas were cultured using a monolayer culture for first few passages, and then a portion of each specimen was cultured in a collagen gel. Immunostaining for synaptophysin or glial fibrillary acidic protein performed on the primary culture revealed the presence of cells expressing synaptophysin and cells expressing glial fibrillary acidic protein. Cells expressing synaptophysin tended to disappear in passage 2, whereas the cells expressing glial fibrillary acidic protein remained. Ultrastructurally, samples in passage 5 obtained from the collagen gel cultures revealed neuron-like cells with prominent nucleoli, cell processes containing dense core vesicles and clear vesicles, and synapse-like structures. By contrast, samples obtained from passage 5 of monolayer cultures failed to reveal ultrastructural neuronal characteristics. These results suggest that spatial cell growth and the presence of collagen, i.e., extracellular matrix, may be necessary to retain neuronal differentiation in a central neurocytoma.
中枢神经细胞瘤是一种具有神经元分化的罕见脑肿瘤。由于该肿瘤数量稀少，对培养的中枢神经细胞瘤细胞的描述不多。最初的几代培养中，使用单层培养法培养了两例中枢神经细胞瘤，然后将每个标本的一部分在胶原凝胶中培养。对原代培养物进行突触素或胶质纤维酸性蛋白免疫染色，结果显示存在表达突触素的细胞和表达胶质纤维酸性蛋白的细胞。表达突触素的细胞在第2代时往往消失，而表达胶质纤维酸性蛋白的细胞仍然存在。在超微结构上，从胶原凝胶培养物中获得的第5代样本显示出具有突出核仁的神经元样细胞、含有致密核心囊泡和清亮囊泡的细胞突起以及突触样结构。相比之下，从单层培养物第5代获得的样本未显示出超微结构的神经元特征。这些结果表明，空间细胞生长以及胶原（即细胞外基质）的存在可能是维持中枢神经细胞瘤神经元分化所必需的。
