Department of Neurosurgery, Seoul National University College of Medicine, Seoul National University, Seoul, Korea.
J Korean Med Sci. 2010 May;25(5):798-803. doi: 10.3346/jkms.2010.25.5.798. Epub 2010 Apr 16.
A seventeen-year-old female patient was admitted with sudden-onset of headache and vomiting. Brain magnetic resonance imaging demonstrated a heterogeneously enhancing tumour in the left lateral ventricle. The tumour was removed and confirmed as a central neurocytoma (CN). For the residual tumour in the left lateral ventricle, gamma knife stereotactic radiosurgery was done at fifteen months after the initial surgery. Tumour recurred in the 4th ventricle at 5 yr after initial surgery. The tumour was removed and proved as a CN. In vitro primary culture was done with both tumours obtained from the left lateral ventricle and the 4th ventricle, respectively. Nestin, a neuronal stem cell marker was expressed in reverse Transcriptase-Polymerase Chain Reaction of both tumors. Both tumours showed different morphology and phenotypes of neuron and glia depending on the culture condition. When cultured in insulin, transferrin selenium and fibronectin media with basic fibroblast growth factors, tumour cells showed neuronal morphology and phenotypes. When cultured in the Dulbeco's Modified Essential Media with 20% fetal bovine serum, tumors cells showed glial morphology and phenotypes. It is suggested that CN has the characteristics of neuronal stem cells and potential to differentiate into mature neuron and glial cells depending on the environmental cue.
一位 17 岁的女性患者因突发性头痛和呕吐入院。脑部磁共振成像显示左侧侧脑室有一个不均匀强化的肿瘤。肿瘤被切除并确认为中枢神经细胞瘤（CN）。对于左侧侧脑室残留的肿瘤，在初次手术后 15 个月进行了伽玛刀立体定向放射外科手术。初次手术后 4 年，肿瘤再次出现在第四脑室。肿瘤被切除并证实为 CN。对分别从左侧脑室和第四脑室获得的两个肿瘤进行了体外原代培养。巢蛋白，一种神经干细胞标志物，在两个肿瘤的逆转录-聚合酶链反应中表达。根据培养条件，两个肿瘤显示出不同的神经元和神经胶质的形态和表型。当在含有碱性成纤维细胞生长因子的胰岛素、转铁蛋白硒和纤维连接蛋白培养基中培养时，肿瘤细胞表现出神经元形态和表型。当在含有 20%胎牛血清的杜尔贝科改良基本培养基中培养时，肿瘤细胞表现出神经胶质形态和表型。提示 CN 具有神经干细胞的特征，并能根据环境线索分化为成熟的神经元和神经胶质细胞。
