Horwood N J, Elliott J, Martin T J, Gillespie M T
St. Vincent's Institute of Medical Research and The University of Melbourne, Department of Medicine, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
Endocrinology. 1998 Nov;139(11):4743-6. doi: 10.1210/endo.139.11.6433.
The osteoclastogenic factor of osteoblastic origin has recently been elucidated as a novel Tumor Necrosis Factor (TNF)-ligand family member, termed osteoclast differentiation factor (ODF). Using a semiquantitative RT-PCR approach, we sought to determine the mRNA expression of ODF and its decoy receptor, osteoprotegerin (OPG), in a selection of osteoblastic cell lines and in response to three factors representative of different signal transduction pathways, vitamin D receptor, protein kinase A or gp130. Each osteotropic agent, either 1,25-(OH)2D3, PTH or IL-11, promoted an increase in the ratio of ODF:OPG, with maximal stimulation occurring at 24 h, 4 h, and 8 h, respectively, and furthermore each was shown to act in a dose-dependent manner. This report establishes that osteoblastic cell lines incapable of supporting osteoclast formation have markedly reduced ODF expression and also illustrates the importance of the relative abundance of ODF compared with the levels of OPG for the induction of osteoclastogenesis.
最近已阐明，成骨细胞来源的破骨细胞生成因子是一种新型肿瘤坏死因子（TNF）配体家族成员，称为破骨细胞分化因子（ODF）。我们采用半定量逆转录聚合酶链反应（RT-PCR）方法，试图确定ODF及其诱饵受体骨保护素（OPG）在一系列成骨细胞系中的mRNA表达情况，以及它们对代表不同信号转导途径的三种因子（维生素D受体、蛋白激酶A或gp130）的反应。每种促骨生长因子，即1,25-二羟维生素D3、甲状旁腺激素（PTH）或白细胞介素-11，均可使ODF:OPG比值升高，最大刺激分别出现在24小时、4小时和8小时，而且每种因子均呈剂量依赖性作用。本报告证实，无法支持破骨细胞形成的成骨细胞系中ODF表达明显降低，同时也说明了与OPG水平相比，ODF的相对丰度对于诱导破骨细胞生成的重要性。
