Hrazdira I, Skorpíková J, Dolníková M
Department of Biophysics, Faculty of Medicine, Masaryk University, Jostova 10, CZ 662 43 Brno, Czech Republic.
Eur J Ultrasound. 1998 Sep;8(1):43-9. doi: 10.1016/s0929-8266(98)00049-4.
The aim of this study was to establish: (i) which phase of the cell cycle is most sensitive to ultrasonic action; and (ii) whether and in which way ultrasound can influence components of the cytoskeleton.
HeLa cell monolayers grown on glass cover-slips in DEM medium were used in all experiments. For proliferation studies, the cell monolayers were trypsinized and the cells were resuspended in fresh medium. The structure of the cytoskeleton was studied by means of the indirect immunofluorescence method. The cells were sonicated by a cw ultrasound of 0.8 MHz at low SA intensities (50, 100 and 500 mW/cm2) for 5 and 10 min.
The analysis of proliferation demonstrated that cells were most sensitive when undergoing M- and S-phases of the cell cycle. The ultrasonically induced disassembly of cytoskeleton components was most marked in microtubules and microfilaments due to depolymerization of basic proteins (tubulin and actin). The reaction of intermediate filaments was distinctly weaker.
In-vitro treatment of tumour cells with low intensity ultrasound results in partial inhibition of proliferation as well as in partial disassembly of all components of the cytoskeleton. Ultrasonically induced changes of the cytoskeleton seem to be non-specific and temporary.
本研究的目的是确定：（i）细胞周期的哪个阶段对超声作用最敏感；（ii）超声是否以及以何种方式能够影响细胞骨架的组成成分。
所有实验均使用在DEM培养基中生长于玻璃盖玻片上的HeLa细胞单层。为进行增殖研究，将细胞单层用胰蛋白酶消化，细胞重悬于新鲜培养基中。通过间接免疫荧光法研究细胞骨架的结构。细胞用0.8MHz的连续波超声在低声强（50、100和500mW/cm²）下处理5分钟和10分钟。
增殖分析表明，细胞在经历细胞周期的M期和S期时最为敏感。由于基础蛋白（微管蛋白和肌动蛋白）的解聚，超声诱导的细胞骨架成分的解聚在微管和微丝中最为明显。中间丝的反应明显较弱。
用低强度超声体外处理肿瘤细胞会导致增殖部分受抑制以及细胞骨架所有成分部分解聚。超声诱导的细胞骨架变化似乎是非特异性的且是暂时的。
