Zellmer S, Reissig D, Lasch J
Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Hollystrasse 1, D06097, Halle, Germany.
J Control Release. 1998 Nov 13;55(2-3):271-9. doi: 10.1016/s0168-3659(98)00059-5.
Reconstructed human skin was prepared from human keratinoblasts. After 1 week of cultivation at the air-liquid interface a stratified layer developed, similar to native human epidermis. Liposomes with an average diameter of 50 nm, made of phosphatidylcholine (PC), phosphatidylserine (PS) and human stratum corneum lipids (hSCL) were applied on top of this culture system. The rate of penetration through the reconstructed human epidermis was 1.38, 0.55 and 0.013 ng lipidh-1cm-2 for PC, hSCL and PS liposomes, respectively. Electron microscopy and confocal laser scanning microscopy showed that PS and hSCL liposomes aggregated at the skin surface, while PC liposomes remained homogeneously dispersed. Fluorescence measurements demonstrated that vesicles, made of native human stratum corneum lipids rapidly mixed with PS liposomes, weakly with hSCL liposomes and did not mix with PC liposomes.
重组人皮由人角质形成细胞制备而成。在气液界面培养1周后，形成了分层结构，类似于天然人表皮。将平均直径为50nm、由磷脂酰胆碱（PC）、磷脂酰丝氨酸（PS）和人角质层脂质（hSCL）制成的脂质体应用于该培养系统顶部。PC、hSCL和PS脂质体透过重组人表皮的速率分别为1.38、0.55和0.013 ng脂质·h⁻¹·cm⁻²。电子显微镜和共聚焦激光扫描显微镜显示，PS和hSCL脂质体在皮肤表面聚集，而PC脂质体保持均匀分散。荧光测量表明，由天然人角质层脂质制成的囊泡与PS脂质体迅速混合，与hSCL脂质体混合较弱，与PC脂质体不混合。
