Zellmer S, Pfeil W, Lasch J
Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Germany.
Biochim Biophys Acta. 1995 Jul 26;1237(2):176-82. doi: 10.1016/0005-2736(95)00100-h.
The interaction of dimyristoylphosphatidylcholine liposomes with the human stratum corneum was investigated by confocal laser scanning microscopy and differential scanning calorimetry. Human skin is characterized by a high autofluorescence. By introducing appropriate optical filters the autofluorescence of the skin was depressed and the penetration profile of fluorescence labelled vesicles was investigated. From optical sectioning it was obvious that neither the vesicles nor the fluorophore N-(lissamine rhodamine B sulfonyl)diacylphophatidylethanolamine (Rho-PE) penetrates in detectable amounts into the human skin. Differential scanning calorimetry of human stratum corneum revealed, that the peak positions of the human stratum corneum specific endothermic transitions at 10 degrees C, 35 degrees C, 50 degrees C, 62 degrees C, 73 degrees C and 81 degrees C did not change significantly after 18 h of non-occlusive vesicle application. However, the enthalpy of the transitions at 35 degrees C, 50 degrees C, 62 degrees C and 73 degrees C, estimated through peak heights increased, relative to the protein related peak at 81 degrees C. A novel transition at 10 degrees C was observed. From these data we conclude that DMPC liposomes do not penetrate intact into the human skin. We deduce, however, that the vesicles disintegrate at the surface of stratum corneum after non-occlusive application. The individual lipid molecules then interact with the lipid barrier of the stratum corneum and penetrate into the latter, which results in an increase of the enthalpy, related to the lipid components of the SC.
通过共聚焦激光扫描显微镜和差示扫描量热法研究了二肉豆蔻酰磷脂酰胆碱脂质体与人角质层的相互作用。人体皮肤具有高自发荧光的特征。通过引入合适的光学滤光片，降低了皮肤的自发荧光，并研究了荧光标记囊泡的渗透情况。从光学切片可以明显看出，囊泡和荧光团N-（丽丝胺罗丹明B磺酰基）二酰基磷脂酰乙醇胺（Rho-PE）都没有以可检测的量渗透到人体皮肤中。人角质层的差示扫描量热法显示，在非封闭性囊泡应用18小时后，人角质层特异性吸热转变在10℃、35℃、50℃、62℃、73℃和81℃的峰值位置没有显著变化。然而，相对于81℃处与蛋白质相关的峰，通过峰高估计的35℃、50℃、62℃和73℃处转变的焓增加。观察到一个新的10℃的转变。从这些数据我们得出结论，DMPC脂质体不会完整地渗透到人体皮肤中。然而，我们推断，在非封闭应用后，囊泡在角质层表面分解。然后单个脂质分子与角质层的脂质屏障相互作用并渗透到其中，这导致与SC脂质成分相关的焓增加。
