Viguera E, Rodríguez A, Hernández P, Krimer D B, Trellez O, Schvartzman J B
Departamento de Biología Celular y del Desarrollo, CIB (CSIC), Velázquez 144, 28006, Madrid, Spain.
Gene. 1998 Sep 14;217(1-2):41-9. doi: 10.1016/s0378-1119(98)00375-8.
We present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecules. The utility of this model is demonstrated with two examples: replication analysis of the plasmids pBR322 and pHH5.8 in Escherichia coli after digestions with EcoRI and HindIII, respectively.
我们提出了一种计算机模型，用于预测通过中性/中性二维（2D）琼脂糖凝胶电泳分析的DNA片段复制中间体（RI）的预期模式。该模型依赖于复制模式（单向或双向）、线性DNA片段的电泳迁移率以及非线性分子三维形状引起的阻滞作用。通过两个例子证明了该模型的实用性：分别用EcoRI和HindIII消化后，对大肠杆菌中的质粒pBR322和pHH5.8进行复制分析。
