Kochkina Z M
Mikrobiologiia. 1986 Nov-Dec;55(6):1045-7.
The DNA of two Bacillus thuringiensis phages was restricted by endonucleases EcoRI and HindIII and the electrophoretic distribution of the fragments in agarose gel was studied. EcoRI was shown to restrict the DNA of phage 1-97A into 8 fragments and the DNA of phage 1-97B into 12 fragments. Restriction with HindIII results in the formation of 22 and 9 fragments for phage 1-97A and phage 1-97B, respectively. The molecular mass of the DNAs determined by summing up EcoRI restricts is 80.87 MDa for phage 1-97A and 32.45 MDa for phage 1-97B.
用核酸内切酶EcoRI和HindIII对两种苏云金芽孢杆菌噬菌体的DNA进行酶切，并研究了片段在琼脂糖凝胶中的电泳分布。结果表明，EcoRI可将噬菌体1-97A的DNA切成8个片段，将噬菌体1-97B的DNA切成12个片段。用HindIII酶切时，噬菌体1-97A和噬菌体1-97B分别形成22个和9个片段。通过对EcoRI酶切片段分子量求和确定，噬菌体1-97A的DNA分子量为80.87 MDa，噬菌体1-97B的DNA分子量为32.45 MDa。
