黄瓜（Cucumis sativus）中十四烷基硫代乙酸和十四烷基硫代丙酸的脂肪酸α-氧化作用

Fatty acid alpha-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (Cucumis sativus).

作者信息

Andersen Borge G I, Slinde E, Nilsson A

机构信息

Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.

出版信息

Biochim Biophys Acta. 1998 Nov 2;1394(2-3):158-68. doi: 10.1016/s0005-2760(98)00113-1.

DOI:10.1016/s0005-2760(98)00113-1

PMID:9795197

Abstract

Fatty acid alpha-oxidation in cucumber (Cucumis sativus) involves enzymatic conversion of long-chain Cn-fatty acids to the C(n-1)-aldehyde and CO2. However, the mechanism of this process is not well understood. In this study, the alpha-oxidation of the fatty acid analogues tetradecylthioacetic acid (TTA) and tetradecylthiopropionic acid (TTP) with a sulphur atom substituting the methylene group in positions 3 and 4, respectively, was investigated and compared to palmitic acid. Both [1-14C]TTA and [1-14C]TTP could be alpha-oxidised in the cucumber subcellular 150000xgmax fraction. [1-14C]TTP was an even better substrate compared to the natural palmitic acid, while [1-14C]TTA was alpha-oxidised to a lower extent. [2-14C]TTA revealed no 14CO2, indicating that only one cycle of alpha-oxidation occurred. TTA was an inhibitor of the palmitic acid alpha-oxidation, and the inhibitory effects were examined.

摘要

黄瓜（Cucumis sativus）中的脂肪酸α-氧化涉及长链Cn脂肪酸酶促转化为C(n-1)-醛和CO₂。然而，这一过程的机制尚未完全了解。在本研究中，对分别用硫原子取代3位和4位亚甲基的脂肪酸类似物十四烷基硫代乙酸（TTA）和十四烷基硫代丙酸（TTP）的α-氧化进行了研究，并与棕榈酸进行了比较。[1-¹⁴C]TTA和[1-¹⁴C]TTP均可在黄瓜亚细胞150000xgmax组分中进行α-氧化。与天然棕榈酸相比，[1-¹⁴C]TTP是更好的底物，而[1-¹⁴C]TTA的α-氧化程度较低。[2-¹⁴C]TTA未检测到¹⁴CO₂，表明仅发生了一轮α-氧化。TTA是棕榈酸α-氧化的抑制剂，并对其抑制作用进行了研究。