Pestonjamasp V K, Burstein S H
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Biochim Biophys Acta. 1998 Nov 2;1394(2-3):249-60. doi: 10.1016/s0005-2760(98)00110-6.
The hypothesis that the capability of agents to mobilize arachidonic acid (AA) could predict increased anandamide (ANA) synthesis in a macrophage cell line has been examined. Lipopolysaccharide (LPS), platelet-activating factor (PAF) and cannabinoids such as Delta9-tetrahydrocannabinol (THC) and anandamide were all found to be agonists for the release of AA and led to increased ANA synthesis in RAW264.7 mouse macrophage cells. Nitric oxide, in contrast, stimulated AA release without raising ANA levels. ANA stimulation of its own synthesis indicates the existence of a positive feedback mechanism. The possible involvement of the CB2 receptor in THC-mediated AA release and ANA synthesis is addressed using the antagonist SR144528. ANA synthesis is also increased by the combination of calcium ionophore and indomethacin, suggesting that ANA is metabolized by a cyclooxygenase in this system. The data imply that ANA could play a role in the response of the immune system to cannabinoids and bacterial endotoxins and that AA mobilization is a predictor for increased ANA synthesis.
研究了关于物质动员花生四烯酸（AA）的能力能否预测巨噬细胞系中花生四烯乙醇胺（ANA）合成增加的假说。脂多糖（LPS）、血小板活化因子（PAF）以及大麻素如Δ9-四氢大麻酚（THC）和花生四烯乙醇胺均被发现是AA释放的激动剂，并导致RAW264.7小鼠巨噬细胞中ANA合成增加。相比之下，一氧化氮刺激AA释放但不提高ANA水平。ANA对其自身合成的刺激表明存在正反馈机制。使用拮抗剂SR144528探讨了CB2受体在THC介导的AA释放和ANA合成中的可能作用。钙离子载体和吲哚美辛的组合也会增加ANA合成，表明在该系统中ANA是由环氧化酶代谢的。数据表明，ANA可能在免疫系统对大麻素和细菌内毒素的反应中起作用，并且AA动员是ANA合成增加的预测指标。
