将可生物降解微粒与MF59作为单纯疱疹病毒2型重组gD的全身佐剂的比较。

A comparison of biodegradable microparticles and MF59 as systemic adjuvants for recombinant gD from HSV-2.

作者信息

Singh M, Carlson J R, Briones M, Ugozzoli M, Kazzaz J, Barackman J, Ott G, O'Hagan D

机构信息

Adjuvant Research Division, Chiron Corporation, Emeryville, CA 94608, USA.

出版信息

Vaccine. 1998 Nov;16(19):1822-7. doi: 10.1016/s0264-410x(98)00179-0.

DOI:10.1016/s0264-410x(98)00179-0

PMID:9795387

Abstract

A recombinant form of glycoprotein D from herpes simplex virus type-2 (gD2) was encapsulated into polylactide-co-glycolide (PLG) microparticles using a previously established solvent evaporation technique. The mean size of the microparticles was about 1 micron and high encapsulation efficiency of the antigen was achieved (70-80%). The microparticles were administered intramuscularly to Balb/C mice and the immune responses were compared with those obtained with the oil in water adjuvant MF59. The serum IgG response to gD2 induced by the microparticles was comparable with that induced by MF59. The serum neutralization titres were also comparable for microparticles and the emulsion. However, the microparticles induced a higher IgG2a isotype response and a more potent serum IFN-gamma response than MF59, suggesting a more Th1 type of response. The MF59 induced higher levels of serum IL-4 and IL-5 cytokines, suggesting a more Th2 type of response.

摘要

采用先前建立的溶剂蒸发技术，将来自2型单纯疱疹病毒的重组糖蛋白D（gD2）包裹于聚乳酸-羟基乙酸共聚物（PLG）微粒中。微粒的平均大小约为1微米，且抗原的包封效率较高（70%-80%）。将这些微粒肌肉注射给Balb/C小鼠，并将免疫反应与使用水包油佐剂MF59所获得的免疫反应进行比较。微粒诱导产生的针对gD2的血清IgG反应与MF59诱导产生的相当。微粒和乳剂的血清中和效价也相当。然而，与MF59相比，微粒诱导产生了更高的IgG2a同种型反应和更强的血清IFN-γ反应，表明是一种更强的Th1型反应。MF59诱导产生了更高水平的血清IL-4和IL-5细胞因子，表明是一种更强的Th2型反应。