Cruz R, Francis L, Díaz-Suárez C M, González-Fraguela M E
Laboratorio de Neurobioquímica, Centro Internacional de Restauración Neurológica, CIREN, La Habana, Cuba.
Rev Neurol. 1998 Apr;26(152):551-4.
The cholinergic neurones of the basal forebrain play an important part in cognitive activity and axotomy of these neurones leads to their retrograde atrophy. The nootropic drug, Cerebrolysin, can protect these neurones from neurodegeneration induced by axotomy. Oxidative damage to biomolecules often occurs in neurodegenerative processes.
To determine whether transection of the septohippocampal bundle (fimbria-fornix, FF) and administration of Cerebrolysin induce alterations in antioxidant mechanisms.
Four groups of rats were studied: Healthy, treated with physiological saline; healthy treated with Cerebrolysin (2.5 ml/kg daily for one week, intraperitoneally); FF-lesioned and pretreated with physiological saline, and with lesions and pretreated with Cerebrolysin. Twenty four hours after the lesion was produced, the activity of the enzymes glutathione S-transferase (GST), glutathione reductase (GRD) and glutathione peroxidase were measured in various cerebral areas.
The lesion caused reduced activity of the three enzymes in the hippocampus, and also in the activity of striatal GRD. Pretreatment with Cerebrolysin did not modify the enzymes in FF-lesioned rats, but did reduce the activity of hippocampal GST in healthy rats.
The results show the sensitivity of enzyme of the glutathione system to denervation of the hippocampus, together with a modest effect of Cerebrolysin in this experimental paradigm.
基底前脑的胆碱能神经元在认知活动中起重要作用，这些神经元的轴突切断会导致其逆行性萎缩。益智药物脑蛋白水解物可保护这些神经元免受轴突切断诱导的神经退行性变。生物分子的氧化损伤常发生在神经退行性变过程中。
确定切断海马隔束（穹窿海马伞，FF）并给予脑蛋白水解物是否会诱导抗氧化机制的改变。
研究了四组大鼠：健康组，给予生理盐水治疗；健康组，给予脑蛋白水解物治疗（每天2.5ml/kg，腹腔注射，持续一周）；FF损伤组，预处理给予生理盐水；FF损伤组，预处理给予脑蛋白水解物。损伤产生24小时后，测量各脑区谷胱甘肽S-转移酶（GST）、谷胱甘肽还原酶（GRD）和谷胱甘肽过氧化物酶的活性。
损伤导致海马中这三种酶的活性降低，纹状体GRD的活性也降低。脑蛋白水解物预处理对FF损伤大鼠的酶活性没有影响，但确实降低了健康大鼠海马中GST的活性。
结果表明谷胱甘肽系统的酶对海马去神经支配敏感，同时在该实验范式中脑蛋白水解物有适度作用。
