Chauhan Shailender Singh, Ojha Sudarshan, Mahmood Akhtar
Department of Biochemistry, Panjab University, Candigarh, India.
Indian J Exp Biol. 2013 Mar;51(3):249-55.
Exposure to fluoride and excessive ethanol consumption has been identified as a serious public health problem in many parts of the world, including India. Thus, the effect of co-exposure to fluoride and ethanol for 3-6 weeks was studied on lipid peroxidation (LPO) and oxidative stress related parameters in the rat brain. After 3 weeks, co-treated animals showed 95% increase in LPO levels compared to control. However, the levels of reduced glutathione, total and protein thiols were decreased. These changes were accompanied by a decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase. Rats exposed to fluoride together with ethanol for 6 weeks resulted in 130% increase in LPO and decrease in the reduced glutathione levels. The activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase were reduced under these conditions. Brain histology revealed excessive lymphocytes, edema and spongeosis in the cortical region after six weeks of fluoride and ethanol treatment. These results suggest that exposure to fluoride together with ethanol enhances lipid peroxidation by affecting antioxidant defence systems in the rat brain.
在包括印度在内的世界许多地区，接触氟化物和过量饮酒已被认定为严重的公共卫生问题。因此，研究了大鼠大脑中氟化物和乙醇共同暴露3至6周对脂质过氧化（LPO）及氧化应激相关参数的影响。3周后，与对照组相比，联合处理的动物LPO水平增加了95%。然而，还原型谷胱甘肽、总硫醇和蛋白质硫醇的水平却下降了。这些变化伴随着超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶活性的降低。氟化物与乙醇共同暴露6周的大鼠，LPO增加了130%，还原型谷胱甘肽水平降低。在这些条件下，超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶的活性均降低。脑部组织学检查显示，经过6周的氟化物和乙醇处理后，皮质区域出现大量淋巴细胞、水肿和海绵样变。这些结果表明，氟化物与乙醇共同暴露通过影响大鼠大脑中的抗氧化防御系统增强了脂质过氧化。
