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[从溶原性培养物和绵羊瘤胃中分离出的牛链球菌噬菌体的生物学特性]

[Biological properties of Streptococcus bovis bacteriophages isolated from lysogenic cultures and sheep rumen].

作者信息

Tarakanov B V

出版信息

Mikrobiologiia. 1976;45(4):695-700.

PMID:979689
Abstract

Biological characteristics of eleven phages for Streptococcus bovis were investigated; seven phage were isolated from ovine rumen and four were virulent mutants of temperate phages of lysogenic cultures. The phages had many properties in common: similar morphology of negative colonies, the identical spectrum of lytic action, related antigens, absolute or high requirement of calcium ions, thermolability, and inactivation by the content of the rumen. Their susceptibility to the inactivating action of acetic acid, urea and temperature was however different. Chloroform and phenol may be used during purification and conservation of the phages.

摘要

对11株牛链球菌噬菌体的生物学特性进行了研究;7株噬菌体从绵羊瘤胃中分离得到,4株是溶原培养物的温和噬菌体的烈性突变体。这些噬菌体有许多共同特性:阴性菌落形态相似、裂解作用谱相同、抗原相关、对钙离子有绝对或高度需求、热稳定性差以及会被瘤胃内容物灭活。然而,它们对乙酸、尿素和温度的灭活作用的敏感性有所不同。在噬菌体的纯化和保存过程中可以使用氯仿和苯酚。

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