通过振荡离心提高活精子的回收率。

Increased recovery of viable spermatozoa through oscillating centrifugation.

作者信息

Ozornek M H, Biefeld P, Jeyendran R S

机构信息

Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.

出版信息

Fertil Steril. 1998 Oct;70(4):712-4. doi: 10.1016/s0015-0282(98)00271-4.

DOI:10.1016/s0015-0282(98)00271-4

PMID:9797103

Abstract

OBJECTIVE

To compare the number and quality of spermatozoa recovered following the standard centrifugation method with those recovered following oscillation of the relative centrifugal field during the centrifugation method.

DESIGN

Prospectively controlled in vitro study.

SETTING

Private andrology laboratory.

PATIENT(S): Seventeen healthy sperm donors.

INTERVENTION(S): Equal halves of the same semen sample were evaluated following the standard centrifugation or following oscillation of the relative centrifugal field during centrifugation.

MAIN OUTCOME MEASURE(S): Number of sperm, sperm motility, progressive sperm motility at 0 hours and after 3-hour of incubation at 5 degrees C in TES and Tris (buffer) (TEST)-yolk.

RESULT(S): The number of sperm, sperm motility, progressive sperm motility at 0 hours and after 3-hour of incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following oscillating centrifugation compared with standard centrifugation.

CONCLUSION(S): Oscillating the relative centrifugal field during centrifugation yields higher number of viable spermatozoa.

摘要