离心脉冲可提高活精子的回收率。
Centrifugal pulsing increases the recovery of viable spermatozoa.
作者信息
Bielfeld P, Acosta V, Jeyendran R S
机构信息
Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.
出版信息
J Assist Reprod Genet. 1998 Nov;15(10):609-11. doi: 10.1023/a:1020385411769.
PURPOSE
Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.
METHODS
A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk results determined the efficacy of the centrifugation techniques.
RESULTS
The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.
CONCLUSIONS
Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.
目的
我们的目的是比较采用标准离心法回收的精子数量和质量与在离心过程中对相对离心场进行脉冲处理后回收的精子数量和质量。
方法
采用前瞻性对照体外研究，使用10名健康精子捐献者的精液。在TEST-卵黄培养基中于5℃孵育0、3和24小时后的精子数量和精子活力决定了离心技术的效果。
结果
与标准离心后回收的精子相比，脉冲离心后回收的精子在TEST-卵黄培养基中于5℃孵育0、3和24小时后的精子数量和精子活力显著更高。
结论
在离心过程中对相对离心场进行脉冲处理可产生更多有活力的精子。
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