二氟甲烷，一种新型且改良的甲烷营养抑制物。
Difluoromethane, a new and improved inhibitor of methanotrophy.
作者信息
Miller LG, Sasson C, Oremland RS
机构信息
U.S. Geological Survey, Menlo Park, California 94025, USA.
出版信息
Appl Environ Microbiol. 1998 Nov;64(11):4357-62. doi: 10.1128/AEM.64.11.4357-4362.1998.
Abstract
Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation by Methylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and this inhibition was reversed by DFM removal. Although a small quantity of DFM was consumed during these incubations, its remaining concentration was sufficiently elevated to sustain inhibition. Methanogenesis in anaerobic soil slurries, including acetoclastic methanogenesis, was unaffected by levels of DFM which inhibit methanotrophy. Low levels of DFM (0.03 kPa) also inhibited nitrification and N2O production by soils. DFM is proposed as an improved inhibitor of CH4 oxidation over acetylene and/or methyl fluoride on the basis of its reversibility, its efficacy at low concentrations, its lack of inhibition of methanogenesis, and its low cost.
摘要
在培养物和土壤中，将二氟甲烷（HFC - 32；DFM）作为甲烷营养的抑制剂与乙炔和氟甲烷进行了比较。发现DFM是荚膜甲基球菌（巴斯德菌）氧化CH4的可逆抑制剂。低水平的DFM（0.03 kPa）添加可阻断土壤中CH4的消耗，去除DFM后这种抑制作用可逆转。尽管在这些培养过程中消耗了少量DFM，但其剩余浓度仍足以维持抑制作用。厌氧土壤泥浆中的产甲烷作用，包括乙酸裂解产甲烷作用，不受抑制甲烷氧化的DFM水平影响。低水平的DFM（0.03 kPa）也抑制土壤中的硝化作用和N2O产生。基于其可逆性、低浓度下的有效性、对产甲烷作用的无抑制性以及低成本，DFM被提议作为一种比乙炔和/或氟甲烷更优的CH4氧化抑制剂。
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