Taylor D E, Rasko D A, Sherburne R, Ho C, Jewell L D
Department of Medical Microbiology and Immunology, Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada.
Gastroenterology. 1998 Nov;115(5):1113-22. doi: 10.1016/s0016-5085(98)70082-4.
BACKGROUND & AIMS: Lewis antigens are expressed by both human gastric epithelial tissue and Helicobacter pylori. We examined Lewis antigens expressed by gastric epithelium and by H. pylori isolated from the corresponding biopsy tissue.
H. pylori Lewis expression was determined by enzyme immunoassays, and immunoelectron microscopy was used to confirm the Lewis antigens on some H. pylori cells and in some biopsy specimens. Histopathology using identical monoclonal antibodies specific for Lewis A, B, X, and Y antigens was used to detect these antigens in 24 gastric biopsy specimens.
We identified Lewis Y in 100%, Lewis X and Lewis B in 95.8%, and Lewis A in 87.5% of biopsy specimens. In H. pylori, 87.5% expressed Lewis Y, 79.2% Lewis X, and 4.2% (one strain) Lewis B. No Lewis A was detected. Antibody specific for Lewis X labeled the bacteria and associated adhesion pedestal. The cagA gene was present in 92% of strains.
There was no direct relationship between Lewis antigen expression by H. pylori and gastric epithelial cells in infected patients. Expression of Lewis X and Lewis Y by H. pylori suggests the possibility of their requirement for establishment and/or maintenance of infection. An immunoelectron micrograph of H. pylori interaction with the gastric epithelial adhesion pedestal suggests a tentative role for Lewis X in the adhesion process.
人类胃上皮组织和幽门螺杆菌均表达Lewis抗原。我们检测了胃上皮以及从相应活检组织中分离出的幽门螺杆菌所表达的Lewis抗原。
采用酶免疫测定法测定幽门螺杆菌的Lewis表达，并利用免疫电子显微镜证实部分幽门螺杆菌细胞和部分活检标本上的Lewis抗原。使用针对Lewis A、B、X和Y抗原的相同单克隆抗体进行组织病理学检查，以检测24份胃活检标本中的这些抗原。
我们在100%的活检标本中鉴定出Lewis Y，95.8%的标本中鉴定出Lewis X和Lewis B，87.5%的标本中鉴定出Lewis A。在幽门螺杆菌中，87.5%表达Lewis Y，79.2%表达Lewis X，4.2%（一株）表达Lewis B。未检测到Lewis A。针对Lewis X的抗体标记了细菌及相关黏附基座。92%的菌株存在cagA基因。
在感染患者中，幽门螺杆菌表达的Lewis抗原与胃上皮细胞之间不存在直接关系。幽门螺杆菌表达Lewis X和Lewis Y表明它们可能参与感染的建立和/或维持。一张幽门螺杆菌与胃上皮黏附基座相互作用的免疫电子显微镜照片表明Lewis X在黏附过程中可能发挥作用。
