Doern G V, Brueggemann A B, Blocker M, Dunne M, Holley H P, Kehl K S, Duval J, Kugler K, Putnam S, Rauch A, Pfaller M A
University of Massachusetts Medical Center, Worcester, USA.
Clin Infect Dis. 1998 Oct;27(4):757-61. doi: 10.1086/514937.
During the past 6 to 7 years, the problem of antimicrobial resistance in Streptococcus pneumoniae has grown dramatically in the United States. Currently, approximately 26.5% of pneumococcal isolates express intermediate levels of resistance to penicillin; approximately 17.5% are highly penicillin resistant. We studied whether clonal relationships exist among current isolates of high-level penicillin-resistant S. pneumoniae (PRSP) in the United States. One hundred forty-seven PRSP isolates recovered in a 30-center surveillance study in the United States during 1994-1995 were characterized with respect to serotype, antimicrobial susceptibility pattern, and pulsed-field gel electrophoresis (PFGE) profile. Only six serotypes were observed among the 147 PRSP isolates examined in this study: 6A, 6B, 9A, 14, 19F, and 23F. One hundred three (70.1%) of the 147 strains were characterized by one of only nine PFGE types; 76 (51.7%) of the 147 isolates were characterized by only four PFGE profiles. Currently in the United States, most PRSP strains are represented by relatively few clonal groups.
在过去6到7年里，肺炎链球菌的抗菌药物耐药性问题在美国急剧增加。目前，约26.5%的肺炎球菌分离株对青霉素呈现中度耐药；约17.5%对青霉素高度耐药。我们研究了美国目前的高水平青霉素耐药肺炎链球菌（PRSP）分离株之间是否存在克隆关系。在1994 - 1995年美国一项30个中心参与的监测研究中收集的147株PRSP分离株，对其血清型、抗菌药物敏感性模式和脉冲场凝胶电泳（PFGE）图谱进行了特征分析。在本研究检测的147株PRSP分离株中仅观察到6种血清型：6A、6B、9A、14、19F和23F。147株菌株中的103株（70.1%）仅由9种PFGE类型中的一种所代表；147株分离株中的76株（51.7%）仅由4种PFGE图谱所代表。目前在美国，大多数PRSP菌株由相对较少的克隆群所代表。
