Yoshida R, Hirakata Y, Kaku M, Takemura H, Tanaka H, Tomono K, Koga H, Kohno S, Kamihira S
Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.
Chemotherapy. 1997 Jul-Aug;43(4):232-8. doi: 10.1159/000239572.
No data on the genetic analysis of penicillin-resistant Streptococcus pneumoniae (PRP) in Japan has been reported. The SmaI restriction endonuclease digested patterns of chromosomal DNAs from 15 PRP serotyped 23F isolated in Japan were analyzed by pulsed-field gel electrophoresis (PFGE). The isolates were genetically heterogeneous and seven different PFGE patterns were identified. Nine strains were also resistant to erythromycin and tetracycline. Four strains revealed resistance to ceftriaxone. The PFGE patterns of some strains isolated in Nagasaki University Hospital were identical to each other and closely resembled those of isolates from three different areas of Japan. These results indicate a need for additional studies by PFGE to determine the possibility of clonal spread in Japan.
日本尚未有关于耐青霉素肺炎链球菌（PRP）基因分析的数据报道。通过脉冲场凝胶电泳（PFGE）分析了从日本分离出的15株血清型为23F的PRP的染色体DNA经SmaI限制性内切酶消化后的模式。这些分离株在基因上具有异质性，鉴定出了7种不同的PFGE模式。9株菌株还对红霉素和四环素耐药。4株菌株显示对头孢曲松耐药。长崎大学医院分离出的一些菌株的PFGE模式彼此相同，且与来自日本三个不同地区的分离株的模式非常相似。这些结果表明需要通过PFGE进行更多研究，以确定在日本克隆传播的可能性。
