RAD3和SSL1基因中的新型突变通过刺激酿酒酵母中短重复序列之间的重组来扰乱基因组稳定性。
Novel mutations in the RAD3 and SSL1 genes perturb genome stability by stimulating recombination between short repeats in Saccharomyces cerevisiae.
作者信息
Maines S, Negritto M C, Wu X, Manthey G M, Bailis A M
机构信息
Department of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA.
出版信息
Genetics. 1998 Nov;150(3):963-76. doi: 10.1093/genetics/150.3.963.
Abstract
Maintaining genome stability requires that recombination between repetitive sequences be avoided. Because short, repetitive sequences are the most abundant, recombination between sequences that are below a certain length are selectively restricted. Novel alleles of the RAD3 and SSL1 genes, which code for components of a basal transcription and UV-damage-repair complex in Saccharomyces cerevisiae, have been found to stimulate recombination between short, repeated sequences. In double mutants, these effects are suppressed, indicating that the RAD3 and SSL1 gene products work together in influencing genome stability. Genetic analysis indicates that this function is independent of UV-damage repair and mutation avoidance, supporting the notion that RAD3 and SSL1 together play a novel role in the maintenance of genome integrity.
摘要
维持基因组稳定性需要避免重复序列之间的重组。由于短重复序列最为丰富，低于一定长度的序列之间的重组受到选择性限制。编码酿酒酵母基础转录和紫外线损伤修复复合体组分的RAD3和SSL1基因的新等位基因，已被发现可刺激短重复序列之间的重组。在双突变体中，这些效应受到抑制，表明RAD3和SSL1基因产物在影响基因组稳定性方面共同发挥作用。遗传分析表明，该功能独立于紫外线损伤修复和突变避免，支持RAD3和SSL1共同在维持基因组完整性中发挥新作用的观点。
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