Bailis A M, Maines S, Negritto M T
Department of Molecular Genetics, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA.
Mol Cell Biol. 1995 Aug;15(8):3998-4008. doi: 10.1128/MCB.15.8.3998.
We have isolated an allele of the essential DNA repair and transcription gene RAD3 that relaxes the restriction against recombination between short DNA sequences in Saccharomyces cerevisiae. Double-strand break repair and gene replacement events requiring recombination between short identical or mismatched sequences were stimulated in the rad3-G595R mutant cells. We also observed an increase in the physical stability of double-strand breaks in the rad3-G595R mutant cells. These results suggest that the RAD3 gene suppresses recombination involving short homologous sequences by promoting the degradation of the ends of broken DNA molecules.
我们分离出了必需的DNA修复和转录基因RAD3的一个等位基因，该等位基因可缓解酿酒酵母中短DNA序列之间重组的限制。在rad3 - G595R突变细胞中，需要短的相同或错配序列之间进行重组的双链断裂修复和基因置换事件受到了刺激。我们还观察到rad3 - G595R突变细胞中双链断裂的物理稳定性有所增加。这些结果表明，RAD3基因通过促进断裂DNA分子末端的降解来抑制涉及短同源序列的重组。
